Immunofluorescence analysis of Retinoic Acid Receptor beta was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Retinoic Acid Receptor beta Rabbit Polyclonal Antibody (PA1811) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Yeast, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues P(429) S V S P S S V E N S G V S Q S P L L Q(448) of mouse RAR beta.|
|Storage buffer||whole serum diluted in PBS|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-811 detects retinoic acid receptor (RAR) beta from human and mouse tissues. This antibody shows slight cross-reactivity to RAR alpha but does not detect RAR gamma.
PA1-811 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~52 kDa protein representing RAR beta from SH-SY5Y (human neuroblastoma) cell extract.
The PA1-811 immunogen is a synthetic peptide corresponding to residues P(429) S V S P S S V E N S G V S Q S P L L Q(448) of mouse RAR beta. This immunizing peptide (Cat. # PEP-005) is available for use in neutralization and control experiments.
Retinoic acid, a metabolite of vitamin A, is necessary for normal organogenesis but acts as a teratogen at high levels during embryonic and fetal development. Retinoic acid functions through its interaction with the nuclear protein, retinoic acid receptor (RAR). RAR belongs to the steroid and thyroid hormone superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. RAR exists as three major subtypes: alpha, beta and gamma. RAR beta is also known as HAP.
The corresponding gene for the retinoic acid receptor beta is NR1B2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Wnt8a and Wnt3a cooperate in the axial stem cell niche to promote mammalian body axis extension.
PA1-811 was used in EMSA to discuss how Wnt8a and Wnt3a contribute to body axis extension
|Cunningham TJ,Kumar S,Yamaguchi TP,Duester G||Developmental dynamics : an official publication of the American Association of Anatomists (244:797)||2015|
Retinoic acid controls body axis extension by directly repressing Fgf8 transcription.
PA1-811 was used in ChIP assay and EMSA to investigate the role of retinoic acid in body axis extension and its mechanism
|Kumar S,Duester G||Development (Cambridge, England) (141:2972)||2014|
A role for Hsp90 in retinoid receptor signal transduction.
PA1-811 was used in western blot to investigate the role of heat shock protein (Hsp90) in retinoid receptor signal transduction
|Holley SJ,Yamamoto KR||Molecular biology of the cell (6:1833)||1995|