Western blot analysis was performed on tissue extract (30 ug lysate) of Rat Brain (Lane 1). The blot was probed with Anti-RARG Rabbit Polyclonal Antibody (Product # PA3-813, 1:250 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 ug/ml, 1:4000 dilution). A 42 kDa band corresponding to RARG was observed in the tissue tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the residues E(40) M L S P S F R G L G Q P D L P K E(57) of the N-terminal end of RAR gamma-1.|
|Storage buffer||whole serum diluted in PBS|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Gel Shift (GS)||1-2 µl|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:250|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
PA3-813 detects recombinant human retinoic acid receptor (RAR) gamma-1. The immunizing sequence is 100% conserved in mouse. This antibody detects RAR gamma-1, but not RAR gamma-2, or either of the alpha or beta forms of RAR.
PA3-813 has been successfully used in Western blot, immunoprecipitation and gel shift procedures. By Western blot, this antibody detects an ~50 kDa protein representing RAR gamma-1 from transiently transfected 293T cells. PA3-813 has been shown to efficiently super shift the human RAR gamma-1/retinoid X receptor (RXR)/RAR response element complex in gel super shift experiments using a number of different response elements.
PA3-813 immunizing peptide corresponds to the N-terminus of RAR gamma-1. This peptide (Cat. # PEP-228) is available for use in neutralization and control experiments.
Retinoic acid, a metabolite of vitamin A, is necessary for normal organogenesis but acts as a teratogen at high levels during embryonic and fetal development. Retinoic acid functions through its interaction with the nuclear protein, retinoic acid receptor (RAR). RAR belongs to the steroid and thyroid hormone superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. RAR exists as three major subtypes: alpha, beta and gamma. Two main isoforms of RAR gamma exist: RAR gamma-1 has been found to be predominantly expressed in skin, while RAR gamma-2 has been found early in embryogenesis and in embryonal carcinoma cells. RAR gamma is also known as RARD.
The corresponding gene for the retinoic acid receptor gamma 1 is NR1B3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The expression of retinoic acid receptor alpha is increased in the granule cells of the dentate gyrus in schizophrenia.
PA3-813 was used in immunohistochemistry to study the expression of retinoic acid receptor alpha in the granule cells of schizophrenic dentate gyrus
|Rioux L,Arnold SE||Psychiatry research (133:13)||2005|