|Tested species reactivity||Human, Mouse|
|Published species reactivity||Bovine, Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues Q(190) K S D Q G V E G P G A T(202) of mouse RXR beta.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Gel Shift (GS)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-815 detects retinoid X receptor (RXR) beta from human and mouse cells as well as transfected human and mouse RXR beta.
PA1-815 has been successfully used in Western blot, immunoprecipitation and gel shift procedures. By Western blot, this antibody detects an ~50 kDa protein representing RXR beta from COS cells transfected with the human gene. Gel shift assays reveal supershifting of vitamin D receptor (VDR)/RXR beta/vitamin D receptor response element (VDRE) complexes when PA1-815 is preincubated with RXR beta prior to addition of VDR.
The PA1-815 immunogen is a synthetic peptide corresponding to residues Q(190) K S D Q G V E G P G A T(202) of mouse RXR beta.
Recombinant human VDR (Cat. # RP-710) and human RXR beta (catalog # RP-812) proteins can be purchased separately for control experiments in Western blot, immunoprecipitation and gel shift procedures.
Retinoid X receptors (RXRs) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. The RXR subfamily consists of at least three similar genes, RXR alpha, RXR beta and RXR gamma all of which control transcription of target genes mediated by retinoids. RXR beta controls expression of many genes that respond to hormones and vitamins, including thyroid hormone, estrogen, retinoids and vitamin D. RXR beta, also known as RCoR-1, controls such a wide array of genes because of its ability to heterodimerize with other hormone receptors including the thyroid hormone receptor (THR), vitamin D receptor (VDR) and retinoic acid receptor (RAR).
The corresponding gene for the retinoid X receptor beta is NR2B2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thymocyte responsiveness to endogenous glucocorticoids is required for immunological fitness.
PA1-815 was used in western blot to study the importance of thymocyte responsiveness to endogenous glucocorticoids in immunological fitness
|Mittelstadt PR,Monteiro JP,Ashwell JD||The Journal of clinical investigation (122:2384)||2012|
Retinoid x receptor agonists increase bcl2a1 expression and decrease apoptosis of naive T lymphocytes.
PA1-815 was used in western blot to identify genes differentially regulated by the RXR agonist AGN194204 during primary antigenic stimulation of naive CD4+ T lymphocytes .
|Rasooly R,Schuster GU,Gregg JP,Xiao JH,Chandraratna RA,Stephensen CB||Journal of immunology (Baltimore, Md. : 1950) (175:7916)||2005|
Thyroid hormone-mediated negative transcriptional regulation of Necdin expression.
PA1-815 was used in ChIP assay to investigate the effect of thyroid hormone on Necdin expression
|Nygård M,Becker N,Demeneix B,Pettersson K,Bondesson M||Journal of molecular endocrinology (36:517)||2006|
The expression of retinoic acid receptor alpha is increased in the granule cells of the dentate gyrus in schizophrenia.
PA1-815 was used in immunohistochemistry to study the expression of retinoic acid receptor alpha in the granule cells of schizophrenic dentate gyrus
|Rioux L,Arnold SE||Psychiatry research (133:13)||2005|
Expression patterns of retinoid X receptors, retinaldehyde dehydrogenase, and peroxisome proliferator activated receptor gamma in bovine preattachment embryos.
PA1-815 was used in immunohistochemistry to profile retinoid X receptors, retinaldehyde dehydrogenase, and peroxisome proliferator activated receptor gamma in bovine preattachment embryos
|Mohan M,Malayer JR,Geisert RD,Morgan GL||Biology of reproduction (66:692)||2002|
Induction of ovarian cancer cell apoptosis by 1,25-dihydroxyvitamin D3 through the down-regulation of telomerase.
PA1-815 was used in EMSA to study the role of 1,25(OH)2VD3 in regulating telomerase activity and cell death in ovarian epithelial cancer cells.
|Jiang F,Bao J,Li P,Nicosia SV,Bai W||The Journal of biological chemistry (279:53213)||2004|
Heterodimeric DNA binding by the vitamin D receptor and retinoid X receptors is enhanced by 1,25-dihydroxyvitamin D3 and inhibited by 9-cis-retinoic acid. Evidence for allosteric receptor interactions.
PA1-815 was used in EMSA assay to investigate the effect of 1,25-(OH)2D3 and 9-cis-RA on the binding of VDR and RXR with VDREs
|Thompson PD,Jurutka PW,Haussler CA,Whitfield GK,Haussler MR||The Journal of biological chemistry (273:8483)||1998|