Invitrogen

Rhodopsin Monoclonal Antibody (RET-P1)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 Published Figure

11 References

View all (14) Rhodopsin antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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Rhodopsin Antibody in Immunohistochemistry (IHC) — Formalin-fixed, paraffin-embedded human brain stained with Rhodopsin antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of neurons.

Rhodopsin Antibody in Western Blot (WB) — Western blot was performed using Rhodopsin Monoclonal Antibody (Product # MA5-11741) and a 40kDa band corresponding to Rhodopsin was observed across the tissues tested except Mouse Heart and Mouse Liver as reported. Also, higher glycosylated form of Rhodopsin ranging between 50-260 kDa were also observed in the tissues along with free floating IgG at ~25kDa. Tissue extracts of Mouse Retina (Lane 1), Mouse Eye (Lane 2), Rat Retina (Lane 3), Rat Eye (Lane 4), Mouse Heart (Lane 5) and Mouse Liver (Lane 6) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Rhodopsin Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue tested owing to their inherent genetic constitution. Relative expression of Rhodopsin was observed in Mouse Retina, Mouse Eye, Rat Retina and Rat Eye in comparison to Mouse Heart and Mouse Liver using Anti-Rhodopsin Monoclonal antibody (Product # MA51-11741) in Western Blot. (doi: 10.1158/2159-8290.CD-14-0337). {RE}

Product Details

MA5-11741

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 8 publications 8 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50 - 1:200	View 1 publication 1 publication
Flow Cytometry (Flow)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Dog, Human, Mouse, Rat
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	RET-P1
Immunogen	Membrane preparation from adult rat retina
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_10980983

Product Specific Information

MA5-11741 targets Rhodopsin in immunofluorescence, immunohistochemistry (paraffin) and Western blot applications and shows reactivity with human, mouse, and rat samples.

The MA5-11741 immunogen is membrane preparation from adult rat retina.

Target Information

Visual pigments such as rhodopsin and porphyropsin are light-absorbing molecules that mediate vision. Rhodopsin consists of an apoprotein, opsin, covalently linked to 11-cis-retinal. This receptor is coupled to the activation of phospholipase C. Porphyropsin consists of opsin covalently linked to 11-cis 3,4-didehydroretinal.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Rai1 frees mice from the repression of active wake behaviors by light.

eLife

Diessler S,Kostic C,Arsenijevic Y,Kawasaki A,Franken P

Published figure using Rhodopsin monoclonal antibody (Product # MA5-11741) in Immunofluorescence

Fri May 26 00:00:00 EDT 2017

Effect of purified murine NGF on isolated photoreceptors of a rodent developing retinitis pigmentosa.

PloS one

Rocco ML,Balzamino BO,Petrocchi Passeri P,Micera A,Aloe L

MA5-11741 was used in flow cytometry and immunocytochemistry to analyze developing retinitis pigmentosa in a rodent after purified murine NGF on isolated photoreceptors

Wed Mar 30 00:00:00 EDT 2016

Retinal Pigment Epithelium Atrophy 1 (rpea1): A New Mouse Model With Retinal Detachment Caused by a Disruption of Protein Kinase C, θ.

Investigative ophthalmology & visual science

Ji X,Liu Y,Hurd R,Wang J,Fitzmaurice B,Nishina PM,Chang B

MA5-11741 was used in immunohistochemistry - paraffin section to utilize a novel mouse model with retinal detachment caused by a disruption of protein kinase C, theta, to study retinal pigment epithelium atrophy 1 (rpea1)

Tue Mar 01 00:00:00 EST 2016

Proliferation of the ciliary epithelium with retinal neuronal and photoreceptor cell differentiation in human eyes with retinal detachment and proliferative vitreoretinopathy.

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie

Ducournau Y,Boscher C,Adelman RA,Guillaubey C,Schmidt-Morand D,Mosnier JF,Ducournau D

MA5-11741 was used in immunohistochemistry to investigate the trans-differentiating capacity of ciliary epithelial cells in human eyes

Thu Mar 01 00:00:00 EST 2012

Identification and functional analysis of the vision-specific BBS3 (ARL6) long isoform.

PLoS genetics

Pretorius PR,Baye LM,Nishimura DY,Searby CC,Bugge K,Yang B,Mullins RF,Stone EM,Sheffield VC,Slusarski DC

MA5-11741 was used in immunohistochemistry to study the function of the vision-specific BBS3 (ARL6) long isoform

Fri Mar 19 00:00:00 EDT 2010

Characterization of a canine model of autosomal recessive retinitis pigmentosa due to a PDE6A mutation.

Investigative ophthalmology & visual science

Tuntivanich N,Pittler SJ,Fischer AJ,Omar G,Kiupel M,Weber A,Yao S,Steibel JP,Khan NW,Petersen-Jones SM

MA5-11741 was used in immunohistochemistry to characterize a canine model of autosomal recessive retinitis pigmentosa

Sun Feb 01 00:00:00 EST 2009

A Critical role for Bim in retinal ganglion cell death.

Journal of neurochemistry

McKernan DP,Cotter TG

MA5-11741 was used in western blot to study the role of Bim in retinal ganglion cell death

Wed Aug 01 00:00:00 EDT 2007

Conversion of mammalian Müller glial cells into a neuronal lineage by in vitro aggregate-culture.

Biochemical and biophysical research communications

Kubota A,Nishida K,Nakashima K,Tano Y

MA5-11741 was used in immunohistochemistry to study the conversion of mammalian Muller glial cells to neuronal cells using in vitro aggregate-culture

Fri Dec 15 00:00:00 EST 2006

Retinal incorporation and differentiation of neural precursors derived from human embryonic stem cells.

Stem cells (Dayton, Ohio)

Banin E,Obolensky A,Idelson M,Hemo I,Reinhardtz E,Pikarsky E,Ben-Hur T,Reubinoff B

MA5-11741 was used in immunohistochemistry to investigate the differentiation of human embryonic stem cells into retinal cells

Wed Feb 01 00:00:00 EST 2006

Late development of vitelliform lesions and flecks in a patient with best disease: clinicopathologic correlation.

Archives of ophthalmology (Chicago, Ill. : 1960)

Mullins RF,Oh KT,Heffron E,Hageman GS,Stone EM

MA5-11741 was used in immunohistochemistry to report a clinical case of Best disease in a senior

Tue Nov 01 00:00:00 EST 2005

Detailed histopathologic characterization of the retinopathy, globe enlarged (rge) chick phenotype.

Molecular vision

Montiani-Ferreira F,Fischer A,Cernuda-Cernuda R,Kiupel M,DeGrip WJ,Sherry D,Cho SS,Shaw GC,Evans MG,Hocking PM,Petersen-Jones SM

MA5-11741 was used in immunohistochemistry to characterize the histopathologic features of retinopathy globe enlarged in chicks

Thu Jan 13 00:00:00 EST 2005

Bioinformatics

Protein Aliases: L opsin; Long Wavelength Sensitive opsin; LWS opsin; MGC138309; MGC138311; OPN 2; opsin 2; opsin 2, rod pigment; Opsin-2; Red Opsin; Rhodopsin; Rhodopsin (retinitis pigmentosa 4, autosomal dominant); Rod Opsin; RP 4

Gene Aliases: CSNBAD1; Noerg1; OPN2; Ops; RHO; RP4

UniProt ID: (Human) P08100, (Rat) P51489, (Mouse) P15409

Entrez Gene ID: (Human) 6010, (Rat) 24717, (Mouse) 212541

Molecular Function: G-protein coupled receptor