Invitrogen

Rhodopsin Monoclonal Antibody (RET-P1)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

1 Published Figure

11 References

View all (12) Rhodopsin antibodies

Datasheet

Datasheet

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Rhodopsin Antibody — Figure 9. Retinal protein and gene expression levels were normal in Rai1 +/- mice. Immunostaining for Rhodopsin protein ( A, B ), the cone protein GNAT2 ( C, D ), the bipolar protein PKCa ( E, F ), the Muller cell protein GFAP ( G, H ), and Calbindin ( I, J ), ChAT ( K, L ), and GABA ( M, N ) markers did not differ between Rai1 +/- ( B, D, F, H, J, L, N ) and Rai1 +/+ mice ( A, C, E, G, I, K, M ). As expected, rhodopsin was mainly localized in outer-segments of rods ( A, B ) and GNAT2 in cone cells ( C, D ). PKCa labeled whole ON bipolar cells with both dendrite and synapse structure ( E, F ). GFAP expression was restricted to the foot of Muller cells not extending to their cell bodies that show no activation in these retinal cells ( G, H ). Calbindin was expressed in horizontal cells (arrows) and some amacrine cells (arrowhead) as well as in ganglion cells located in the GCL ( I, J ). Three strata of connections (star) were well labeled by calbindin in both genotypes in the layer connecting the INL and GCL layers. Both ChAT ( K, L ) and GABA ( M, N ) labeled subpopulations of amacrine cells (arrowheads) and different strata of connections (star) in a pattern similar for Rai1 +/- ( L, N ) and Rai1 +/+ ( K, M ) mice. White horizontal bars represents 50 um in A, B, E, F, G, and H and 100 um in C, D, and I through M. OS: outer-segments; ONL: outer-nuclear layer; INL: inner-nuclear layer; GCL: ganglion cell layer; RHO: rhodopsin in green; GNAT2: G-protein subunit alpha transducin

Rhodopsin Antibody in Immunohistochemistry (IHC) — Formalin-fixed, paraffin-embedded human brain stained with Rhodopsin antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of neurons.

Rhodopsin Antibody in Western Blot (WB) — Western blot analysis was performed on tissue extract (30 µg lysate) of Mouse retina (Lane 1), Rat eye (Lane 2) and Mouse liver (Lane 3). The blot was probed with Anti-Rhodopsin Monoclonal Antibody (RET-P1) (Product # MA5-11741, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 40 kDa band corresponding to Rhodopsin was detected in relevant tissue samples (retina and eye) with no detection in liver tissue sample.

Rhodopsin Antibody in Relative expression — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue models owing to their inherent genetic constitution. Expression of Rhodopsin was observed specifically in Mouse retina and Rat eye using Rhodopsin Monoclonal Antibody (RET-P1) (Product # MA5-11741) in western blot. {RE}

Product Details

Tested Applications

Dilution

Immunofluorescence (IF)

Assay Dependent

Immunohistochemistry (Paraffin) (IHC (P))

1:50 - 1:200

Western Blot (WB)

1:1000

Published Applications

Immunofluorescence (IF)

See 1 publication below

Flow Cytometry (Flow)

See 1 publication below

Immunohistochemistry (Paraffin) (IHC (P))

See 1 publication below

Immunohistochemistry (IHC)

See 7 publications below

Western Blot (WB)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Dog, Human, Mouse, Rat

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

RET-P1

Immunogen

Membrane preparation from adult rat retina

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_10980983

Product Specific Information

MA5-11741 targets Rhodopsin in immunofluorescence, immunohistochemistry (paraffin) and Western blot applications and shows reactivity with human, mouse, and rat samples.

The MA5-11741 immunogen is membrane preparation from adult rat retina.

Target Information

Rhodopsin is a glycoprotein which is a visual pigment in mammals, birds, amphibians, and reptiles. The rhodopsin protein is present on the photoreceptor cells in the retina.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: autosomal dominant; autosomal dominant); L opsin; Long Wavelength Sensitive opsin; LWS opsin; opsin 2; opsin 2, rod pigment; Opsin-2; Red Opsin; retinitis pigmentosa 4; Rhodopsin; rhodopsin (opsin 2; Rhodopsin (retinitis pigmentosa 4, autosomal dominant); Rod Opsin; rod pigment; rod pigment) (retinitis pigmentosa 4

Gene Aliases: CSNBAD1; Noerg1; OPN2; Ops; RHO; RP4

UniProt ID: (Human) P08100, (Rat) P51489, (Mouse) P15409

Entrez Gene ID: (Human) 6010, (Rat) 24717, (Mouse) 212541

Molecular Function: G-protein coupled receptor receptor