Immunohistochemical analysis of S100 Astrocyte Marker in rat brain tissue (30m thick coronal sections) in free floating IHC using a S100 Astrocyte Marker monoclonal antibody (Product # MA1-22734) at a dilution of 1:1000 with an Alexafluor 488 secondary antibody at a dilution of 1:2000. Images show neuron body and processes: 20x objective.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Full length human S100 protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunohistochemistry - Free Floating (IHC (Free))||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-22734 detects S100 in human samples.
MA1-22734 has been successfully used in ELISA, immunohistochemistry (FFPE) and Western blot procedures.
The MA1-22734 immunogen is full length human S100 protein.
S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. Immunoreactive S100 protein localizes in the cytoplasm and nuclei of astrocytes and Schwann's cells. Ganglion cells do not stain for S100 protein. S100 can be detected in almost all benign naevi and malignant melanocytic tumours of the skin, and in Langerhans cells in the skin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.