Invitrogen

SLC2A5 Monoclonal Antibody (14C8)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

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Datasheet

Questions & Answers

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SLC2A5 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of SLC2A5 using anti-SLC2A5 monoclonal antibody (Product # MA1-036) (shown in green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing SLC2A5 (Product # MA1-036), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody (Product # 35503) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.

SLC2A5 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry was performed on cancer biopsies of deparaffinized human testis and hepatic carcinoma tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes at 95°C. Following antigen retrieval tissues were blocked in 10% normal goat serum (Product # 31873) for 20 minutes at room temperature. Tissues were then probed at a dilution of 1:800 with a mouse monoclonal antibody recognizing SLC2A5 (Product # MA1-036) overnight at 4°C in a humidified chamber. Detection was performed using a goat anti-mouse HRP secondary antibody followed by colorimetric detection using DAB substrate. Tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 400X magnification. Results demonstrate membrane/cytoplasmic localization of SLC2A5.

SLC2A5 Antibody in Western Blot (WB) — Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of Daudi (Lane 1), THP-1 (Lane 2), U-937 (Lane 3), MCF7 (Lane 4), HepG2 (Lane 5), Caco2 (Lane 6), Caco2 treated with Forskolin (50uM for 4 days) (Lane 7) and tissue extracts of Mouse Testis (Lane 8). The blot was probed with Anti-SLC2A5 Monoclonal Antibody (Product # MA1-036, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). High molecular weight corresponding to the glycosylated forms of SLC2A5 were observed in all the cell lines tested except MCF7 and HepG2. An 5kDa band corresponding to SLC2A5 was observed in Mouse Testis and the protein was also observed to be induced upon treatment of Caco2 cells with Forskolin.

SLC2A5 Antibody in Relative expression — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lysates owing to their inherent genetic constitution. Relatively high expression of SLC2A5 was observed in all cell lysates tested in comparison to lower expression in MCF7 and HepG2 using product (Product # MA1-036) in western blot. {RE}

SLC2A5 Antibody in Cell treatment — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Western blot analysis of SLC2A5 using with Anti-SLC2A5 Monoclonal Antibody (Product # MA1-036) shows an increase in SLC2A5 upon treatment of Caco2 cells with Forskolin. {TM}

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:100

Immunofluorescence (IF)

1:100

Immunohistochemistry (IHC)

1:800

Western Blot (WB)

1:1000

Product Specifications

Species

Human, Mouse

Expression System

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

14C8

Immunogen

Protein expressed in 293T cell transfected with human SLC2A5 expression vector.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

RRID

AB_11152117

Product Specific Information

This antibody requires overnight incubation in a Western blot for optimal performance. Do not use this antibody in a Fast Western blotting procedure.

Target Information

Cytochalasin B-sensitive carrier. Seems to function primarily as a fructose transporter.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Fructose transporter; Glucose transporter type 5, small intestine; glucose transporter-like protein 5; GLUT-5; RP5-963K15.1; solute carrier family 2 (facilitated glucose/fructose transporter), member 5; Solute carrier family 2, facilitated glucose transporter member 5; testicular tissue protein Li 81

Gene Aliases: AI526984; GLUT-5; GLUT5; SLC2A5; Slc5a

UniProt ID: (Human) P22732, (Mouse) Q9WV38

Entrez Gene ID: (Human) 6518, (Mouse) 56485

Function(s)