|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Chicken|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptides corresponding to residues L(18) G W K Q G D E E E K W A E K(32) and amino acids S(315) N V N R N S T I E N T R R H I(330) of human SMaD1.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody is predicted to react with chicken, chimpanzee, invertebrates, most mammals, porcine, xenopus and zebrafish samples
SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily (1). SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, SMAD2, SMAD5, SMAD8 and SMAD9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and SMAD7). SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bone morphogenetic protein 4 supports the initial differentiation of hen (Gallus gallus) granulosa cells.
PA1-41238 was used in western blot to study the mechanisms underlying the promotion of initial hen granulosa cell differentiation by BMP-4
|Kim D,Ocón-Grove O,Johnson AL||Biology of reproduction (88:null)||2013|