|Tested species reactivity||Fish, Human, Mouse, Rat, Xenopus, Zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, conjugated to maleimide-activated KLH, corresponding to residues K(1354) E D V E K A K K R R G R P P A E(1370) of SMARCA2, with an N-terminal added cysteine|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1 ug/ml|
|Immunofluorescence (IF)||1 µg/ml|
|Western Blot (WB)||0.15 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-25448 detects SMARCA2 from human samples. PA1-25448 is expected to cross react with mouse (100% conserved), rat (100% conserved), Xenopus laevis (88% conserved), zebrafish (87% conserved), and chicken (94% conserved) due to sequence homology.
PA1-25448 has been successfully used in Western blot procedures.
The PA1-25448 immunogen is a synthetic peptide, conjugated to maleimide-activated KLH, corresponding to residues K(1354) E D V E K A K K R R G R P P A E(1370) of SMARCA2, with an N-terminal added cysteine.
The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Two transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.