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SRD5A1 Antibody (PA5-120297) in WB
Western blot analysis of SRD5A1 in extracts of various cell lines. Samples were incubated with SRD5A1 Polyclonal antibody (Product # PA5-120297) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-120297
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human SRD5A1 (NP_001038.1).
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2913869
Product Specific Information
Positive test controls include: Mouse brain, Mouse liver, Mouse testis, Rat liver. The target is usually found in the following locations: Endoplasmic reticulum membrane, Microsome membrane, Multi-pass membrane protein.
Immunogen sequence: LIYPFLMRGG KPMPLLACTM AIMFCTCNGY LQSRYLSHCA VYADDWVTDP RFLIGFGLWL TGMLINIHSD HILRNLRKPG DTGYKIPRGG LFEYVTAANY F
Target Information
Steroid 5alpha-Reductase is an important enzyme in androgen physiology because it catalyzes the conversion of testosterone into the more potent 5alpha-dihydro-testosterone, which mediates androgen effects on target tissues. The enzyme exists as two isoforms: type 1, which is expressed mainly in the skin; and type 2, which is expressed mainly in the prostate. In cultured human skin cells, 5alpha-Reductase 1 shows heterogeneity of protein, and has different levels of transcriptional and translational expression. 5alpha-Reductase 1 is expressed in all portions of the hair follicle, whereas 5alpha-Reductase 2 is expressed only in mesenchymal portions. In addition, 5alpha-Reductase 1 is mainly expressed in human breast carcinoma and may play a role in the in situ production and actions of the potent androgen 5alpha-dihydrotestosterone, including inhibition of cancer cell proliferation in hormone-dependent human breast carcinoma. The 5alpha-Reductase-3alpha-hydroxysteroid dehydrogenase complex is present in the human brain, suggesting that the complex may be involved in the synthesis of neuroactive steroids or the catabolism of neurotoxic steroids.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3-oxo-5-alpha-steroid 4-dehydrogenase 1; S5AR 1; SR type 1; steroid 5 alpha-reductase 1; Steroid 5-alpha-reductase 1; steroid-5-alpha-reductase 1; type 1 5alpha-reductase
Gene Aliases: 0610031P22Rik; 4930435F02Rik; S5AR 1; Srd5a-1; Srd5a1
UniProt ID: (Mouse) Q68FF9, (Rat) P24008
Entrez Gene ID: (Mouse) 78925, (Rat) 24950
lipid metabolic process
steroid biosynthetic process
androgen biosynthetic process
sex differentiation
steroid metabolic process
androgen metabolic process
cell differentiation
progesterone metabolic process
response to drug
oxidation-reduction process
urogenital system development
liver development
male gonad development
cellular response to starvation
response to organic cyclic compound
response to muscle activity
diterpenoid metabolic process
spinal cord development
hippocampus development
thalamus development
hypothalamus development
pituitary gland development
cerebral cortex development
male genitalia development
female genitalia development
response to follicle-stimulating hormone
response to estradiol
cellular response to insulin stimulus
response to testosterone
serotonin metabolic process
circadian sleep/wake cycle, REM sleep
response to estrogen
male sex differentiation
bone development
response to growth hormone
response to fungicide
cellular response to cAMP
cellular response to growth factor stimulus
cellular response to estradiol stimulus
cellular response to testosterone stimulus
cellular response to organic cyclic compound
cellular response to dexamethasone stimulus
cellular response to epinephrine stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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