Immunofluorescent analysis of Securin in HeLa cells using a Securin recombinant rabbit monoclonal antibody (Product # 700791) at a dilution of 2ug/ml followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000. Cells were fixed using 4% paraformaldehyde. The cytoplasmic localization of Securin is shown in green, while nuclei were stained using SlowFade GOLD with DAPI (Product # S36938), shown in blue.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A recombinant protein corresponding to amino acids 1-202.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 ug/ml|
|Immunofluorescence (IF)||2 ug/ml|
|Western Blot (WB)||1-3µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
This antibody is predicted to react with bovine based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Pituitary tumor-transforming gene 1 (PTTG-1) is an oncogene expressed in normal tissues containing proliferating cells and in tumors. PTTG is downstream of the MAP kinase signaling cascade and is phosphorylated at Ser162 by MAPK in vitro. Phosphorylation plays an essential role in PTTG's transactivation function. The PTTG protein contains a DNA-binding domain, and it activates transcription of the c-Myc oncogene by binding to the promoter to form a complex. Studies have demonstrated that expression of human PTTG correlates with cell proliferation in a cell cycledependent manner, and the association of human PTTG with Ku, the regulatory subunit of DNA-dependent protein kinase, is disrupted when a DNA double-strand breaks occurs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Potential new chemotherapy strategy for human ovarian carcinoma with a novel KSP inhibitor.
700791 was used in western blot to describe the anti-cancer effects of KPYB10602.
|Takenaga M,Yamamoto Y,Takeuchi T,Ohta Y,Tokura Y,Hamaguchi A,Asai D,Nakashima H,Oishi S,Fujii N||Biochemical and biophysical research communications (463:222)||2015|