|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, KLH conjugated, corresponding to the 3rd cytoplasmic domain of human 5-HT2C receptor.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.7|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-24507 detects 5-HT2C receptor in human samples.
PA1-24507 has been successfully used in immunohistochemistry procedures.
The PA1-24507 immunogen is synthetic peptide, KLH conjugated, corresponding to the 3rd cytoplasmic domain of human 5-HT2C receptor. Sequence identity/similarity to mouse: 55/65%. rat: 60/70%.
5-HT2C, a Serotonin Receptor previously termed 5-HT1C, activates phospholipase C, resulting in a transient increase in intracellular calcium and activation of protein kinase C. Mutant mice lacking functional 5-HT2C receptor are overweight due to abnormal feeding behavior and are prone to epileptic seizures. Therefore, agonists of the 5-HT2C receptor such as m-chlorophenylpiperazine (mCPP) may be potential drugs for the treatment of obesity. However, mCPP also has unpleasant side effects such as anxiety and nausea in humans. Most psychedelic drugs bind 5-HT2A and 5-HT2C receptors, and their hallucinogenicity correlates with binding affinity. Expression of the 5-HT2C receptor has been reported predominantly in brain, but also in spinal cord and lung. ESTs have been isolated from human, mouse, and rat brain libraries.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.