|Tested species reactivity||Human|
|Published species reactivity||Not Applicable, Guinea pig|
|Host / Isotype||Mouse / IgM|
|Immunogen||Immunoprecipitate obtained after immunodiffusion of MAb 19-9 and mucins isolated from an ovarian cyst of a 0Le(a+b-) patient.|
|Purification||Size-exclusion chromatography, Ammonium sulfate precipitation|
|Storage buffer||TBS, pH 8|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||10µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For IHC (P), heat mediated antigen retrieval in sodium citrate is recommended.
Lewis A (type 1 chain) is expressed in colonic epithelial cells and may be useful for detection of gastrointestinal tumors, pancreatic cancer, and colorectal tumors. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
High-throughput carbohydrate microarray profiling of 27 antibodies demonstrates widespread specificity problems.
MA1-19211 was used in ELISA to study the specificity of a range of antiglycan antibodies
|Manimala JC,Roach TA,Li Z,Gildersleeve JC||Glycobiology (17:17C)||2007|
|Guinea pig||10 ug/ml||
Conjunctival M cells selectively bind and translocate Maackia amurensis leukoagglutinin.
MA1-19211 was used in immunohistochemistry to investigate the presence of conjunctival M cells in the guinea pig conjunctival follicle-associated epithelium and its involvement in mucosal immune response
|Meagher CK,Liu H,Moore CP,Phillips TE||Experimental eye research (80:545)||2005|