Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is skeletal muscle tissue.
MA1-37021 is highly specific and shows no cross-reaction with smooth muscle actin. This antibody reacts with sarcomeric actins of normal tissues and neoplasms derived from such tissues (i.e. rhabdomyosarcomas).
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Protein Aliases: ACTA1; Actin, alpha skeletal muscle; Actin, alpha skeletal muscle, intermediate form; Actin, aortic smooth muscle; Actin, aortic smooth muscle, intermediate form; Alpha-actin-1; Alpha-actin-2; alpha-cardiac actin; Cell growth-inhibiting gene 46 protein; nemaline myopathy type 3
Gene Aliases: AAT6; ACTA; ACTA1; ACTA2; ACTSA; ACTVS; ASMA; CFTD; CFTD1; CFTDM; GIG46; MPFD; MYMY5; NEM1; NEM2; NEM3; SHPM