Western blot detection of SmarcAL1 in 5µg of HeLa nuclear extract (lane 1), HeLa (lane 2), A431 (lane 3), Jurkat (lane 4), and 293 (lane 5). Samples were separated on a SDS-PAGE, transferred onto nitrocellulose, and probed using a SmarcAL1 polyclonal antibody (Product # PA1-31918) at a dilution of 1:500. Signal was detected using standard techniques. SmarcAL1 is the band seen between the 97 and 116kD markers.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||synthetic peptide SNADQRPHDSHSFQAKGC, corresponding to amino acids 80-96 of Human SmarcAL1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-31918 detects SmarcAL1 from human samples.
PA1-31918 has been successfully used in Western blot applications.
The PA1-31918 immunogen is: Synthetic peptide: SNADQRPHDSHSFQAKGC, corresponding to amino acids 80-96 of Human SmarcAL1.
The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.