|Tested species reactivity||Fruit fly, Human, Pig, Rabbit, Rat|
|Published species reactivity||Dog, Fruit fly, Hamster, Mouse, Human|
|Host / Isotype||Mouse / IgG3, kappa|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Immunomicroscopy (IM)||Assay Dependent|
|Western Blot (WB)||0.5-1.0 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The sm (Smith) is an autoantigen on a series of particles composed of RNA and protein. Unlike RNP, the sm antigen is resistant to RNase, but partially sensitive to trypsin. It is involved in the pathogenesis of Systemic lupus erythematosus (SLE). Antibodies to sm are found in 20-30% of patients with SLE.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mutation of a U2 snRNA gene causes global disruption of alternative splicing and neurodegeneration.
MA5-13446 was used in immunoprecipitation to study the role of a mutation of a U2 snRNA gene in global disruption of alternative splicing and neurodegeneration
|Jia Y,Mu JC,Ackerman SL||Cell (148:296)||2012|
PRPF mutations are associated with generalized defects in spliceosome formation and pre-mRNA splicing in patients with retinitis pigmentosa.
MA5-13446 was used in immunoprecipitation to investigate the associates of PRPF mutations with defects in pre-mRNA splicing in patients with retinitis pigmentosa
|Tanackovic G,Ransijn A,Thibault P,Abou Elela S,Klinck R,Berson EL,Chabot B,Rivolta C||Human molecular genetics (20:2116)||2011|
Functional association of the Microprocessor complex with the spliceosome.
MA5-13446 was used in immunoprecipitation to investigate the molecular basis for intronic miRNA processing
|Kataoka N,Fujita M,Ohno M||Molecular and cellular biology (29:3243)||2009|
Restoration of SMN function: delivery of a trans-splicing RNA re-directs SMN2 pre-mRNA splicing.
MA5-13446 was used in immunoprecipitation to study the restoration of survival motor neuron function by a redirection of SMN2 pre-mRNA splicing
|Coady TH,Shababi M,Tullis GE,Lorson CL||Molecular therapy : the journal of the American Society of Gene Therapy (15:1471)||2007|
Gemin8 is a novel component of the survival motor neuron complex and functions in small nuclear ribonucleoprotein assembly.
MA5-13446 was used in immunoprecipitation to investigate the role of gemin8 in the survival motor neuron complex and its function in small nuclear ribonucleoprotein assembly
|Carissimi C,Saieva L,Baccon J,Chiarella P,Maiolica A,Sawyer A,Rappsilber J,Pellizzoni L||The Journal of biological chemistry (281:8126)||2006|
pICln inhibits snRNP biogenesis by binding core spliceosomal proteins.
MA5-13446 was used in immunoprecipitation to study the mechanism for the inhibitory effect of pICln on snRNP biogenesis
|Pu WT,Krapivinsky GB,Krapivinsky L,Clapham DE||Molecular and cellular biology (19:4113)||1999|
The inability of fully grown germinal vesicle stage oocyte cytoplasm to transcriptionally silence transferred transcribing nuclei.
MA5-13446 was used in immunocytochemistry to evaluate the utility of fully grown germinal vesicle stage oocyte cytoplasm in somatic cell nuclear transfer experiments
|Fulka H,Novakova Z,Mosko T,Fulka J||Histochemistry and cell biology (132:457)||2009|
|Fruit fly||Not Cited||
Arginine methyltransferase Capsuleen is essential for methylation of spliceosomal Sm proteins and germ cell formation in Drosophila.
MA5-13446 was used in immunocytochemistry to study the essential role of protein arginine methyltransferase in protein localization in germ cells
|Anne J,Ollo R,Ephrussi A,Mechler BM||Development (Cambridge, England) (134:137)||2007|
Tim50a, a nuclear isoform of the mitochondrial Tim50, interacts with proteins involved in snRNP biogenesis.
MA5-13446 was used in immunocytochemistry to study the role of Tim50a in snRNP biogenesis.
|Xu H,Somers ZB,Robinson ML,Hebert MD||BMC cell biology (6:null)||2005|
A key role for stress-induced satellite III transcripts in the relocalization of splicing factors into nuclear stress granules.
MA5-13446 was used in immunocytochemistry to study the role of stress-induced satellite III transcripts in the relocalization of splicing factors into nuclear stress granules
|Metz A,Soret J,Vourc'h C,Tazi J,Jolly C||Journal of cell science (117:4551)||2004|
ELL and EAF1 are Cajal body components that are disrupted in MLL-ELL leukemia.
MA5-13446 was used in immunocytochemistry to study the disruption of Cajal body components ELL and EAF1 in MLL-ELL leukemia
|Polak PE,Simone F,Kaberlein JJ,Luo RT,Thirman MJ||Molecular biology of the cell (14:1517)||2003|
Mutations in splicing factor PRPF3, causing retinal degeneration, form detrimental aggregates in photoreceptor cells.
MA5-13446 was used in immunohistochemistry to investigate the role of mutations in splicing factor PRPF3 in retinal degeneration of photoreceptor cells
|Comitato A,Spampanato C,Chakarova C,Sanges D,Bhattacharya SS,Marigo V||Human molecular genetics (16:1699)||2007|
Myosin16b: The COOH-tail region directs localization to the nucleus and overexpression delays S-phase progression.
MA5-13446 was used in immunohistochemistry to study the role of the myosin16b COOH-tail region in localization to the nucleus and S-phase progression
|Cameron RS,Liu C,Mixon AS,Pihkala JP,Rahn RJ,Cameron PL||Cell motility and the cytoskeleton (64:19)||2007|
Subcellular localization of protein kinase C delta and epsilon affects transcriptional and post-transcriptional processes in four-cell mouse embryos.
MA5-13446 was used in immunohistochemistry to study the role of protein kinase C delta and epsilon subcellular localization on transcriptional and post-transcriptional processes in mouse preimplantation development
|Dehghani H,Reith C,Hahnel AC||Reproduction (Cambridge, England) (130:453)||2005|