|Tested species reactivity||Human, Mouse|
|Published species reactivity||Non-human primate, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from an internal region of human Smurf1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Members of the transforming growth factor-beta (TGFB) family signal through type I and type II serine/threonine-kinase receptors, which in turn activate the SMAD signaling pathway. Bone morphogenetic protein (BMP) receptors target SMAD1, SMAD5, and SMAD8, whereas receptors for activin and TGFB (e.g., ACVR1 and TGFBR1, respectively) target SMAD2 and SMAD3. Phosphorylation of these receptor-regulated SMADs induces their association with the common-partner SMAD, SMAD4. Smurf1, a HECT domain E3 ubiquitin ligase, regulates cell polarity and protrusive activity and is required to maintain the transformed morphology and motility of a tumor cell. Atypical protein kinase C-zeta (PKC2), an effector of the Cdc42/Rac1-PAR6 polarity complex, recruits Smurf1 to cellular protrusions, where it controlled the local level of RhoA. Smurf1 thus links the polarity complex to degradation of RhoA in lamellipodia and filopodia to prevent RhoA signaling during dynamic membrane movements.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Human||Not Cited||Differential gene expression in normal esophagus and Barrett's esophagus.||Wang J,Qin R,Ma Y,Wu H,Peters H,Tyska M,Shaheen NJ,Chen X||Journal of gastroenterology (44:897)||2009|
||The four-and-a-half LIM domain protein 2 regulates vascular smooth muscle phenotype and vascular tone.||Neuman NA,Ma S,Schnitzler GR,Zhu Y,Lagna G,Hata A||The Journal of biological chemistry (284:13202)||2009|
|Non-human primate||Not Cited||The four-and-a-half LIM domain protein 2 regulates vascular smooth muscle phenotype and vascular tone.||Neuman NA,Ma S,Schnitzler GR,Zhu Y,Lagna G,Hata A||The Journal of biological chemistry (284:13202)||2009|