|Tested species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues E(355) T Q R T L L N G D L Q T S I(369) of human SSTR2A.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:2500|
|Western Blot (WB)||2-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 2 publications below|
PA3-109 detects Somatostatin Receptor 2A in human and rat samples.
PA3-109 has been used successfully in Western blot, immunocytochemistry, and immunohistochemistry procedures. In Western blot analysis of SSTR2A transfected HEK-293 cells this antibody detects a ~62-72 kDa protein representing SSTR2A.
The PA3-109 immunogen is a synthetic peptide corresponding to residues E(355) T Q R T L L N G D L Q T S I(369) of human SSTR2A.
Somatostatin Receptor Type 2 (SST2) expression has been reported in adrenal, aorta, brain, breast, eye, gastrointestinal tract, pancreas, prostate, spinal cord, and thyroid. ESTs have been isolated from brain, eye, head/neck, kidney, lymph node, pineal, and prostate libraries.
G-protein Coupled Receptors (GPCRs) comprise one of the largest families of signaling molecules with more than a thousand members currently predicted to exist. All GPCRs share a structural motif consisting of seven membrane-spanning helices, and exist in both active and inactive forms. An array of activating ligands participate in the conformation of GPCRs which leads to signaling via G-proteins and downstream effectors. Ongoing studies have also shown the vast series of reactions which participate in the negative regulation of GPCRs. This "turn-off" activity has tremendous implications for the physiological action of the cell, and continues to drive pharmacological research for new drug candidates. Two blockbuster drugs which have been developed as GPCR-targeted pharmaceuticals are Zyprexa (Eli Lilly) and Claritin (Schering-Plough) which have multi-billion dollar shares of the mental health and allergy markets, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
SSTR2-based reporters for assessing gene transfer into non-small cell lung cancer: evaluation using an intrathoracic mouse model.
PA3-109 was used in immunohistochemistry to investigate the efficiency of SSTR2-based reporters for gene transfer evaluation in non-small cell lung cancer
|Singh SP,Han L,Murali R,Solis L,Roth J,Ji L,Wistuba I,Kundra V||Human gene therapy (22:55)||2011|
Tumor-induced hypophosphatemic rickets in an adolescent boy--clinical presentation, diagnosis, and histological findings in growth plate and muscle tissue.
PA3-109 was used in immunohistochemistry to investigate a case of tumor-induced hypophosphatemic rickets
|Haeusler G,Freilinger M,Dominkus M,Egerbacher M,Amann G,Kolb A,Schlegel W,Raimann A,Staudenherz A||The Journal of clinical endocrinology and metabolism (95:4511)||2010|