Western blot analysis was performed on whole cell extracts (20 ug lysate) of Hep G2 (Lane 1), PC-3 (Lane 2), MCF7 (Lane 3), Mouse Heart (lane 4), HEK-293 (lane 5), HeLa (lane 6) and Mouse Kidney (lane 9). The blots were probed with Anti-Somatostatin Receptor 5 Rabbit Polyclonal Antibody (Product# PA3112, 1:4000-1:6000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 ug/ml, 1:2500 dilution). Two bands ~ 42 and 39 kDa corresponding to Somatostatin Receptor 5 was observed across cell lines and tissues tested except for HEK-293. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product# SLF2000S). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues Q(344) E A T R P A H R A A A N G L M Q T S K L(364) of human SSTR5.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:2500|
|Western Blot (WB)||1:4000-1:6000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
PA3-112 detects the Somatostatin Receptor 5 in human samples.
PA3-112 has been used successfully in Western blot, immunocytochemistry, and immunohistochemistry procedures. In Western blot analysis of SSTR5 transfected HEK-293 cells this antibody detects a ~54-63 kDa protein representing SSTR5.
The PA3-112 immunogen is a synthetic peptide corresponding to residues Q(344) E A T P P A H R A A A N G L M Q T S K L(364) of human SSTR5.
Somatostatin Receptor Type 5 (SSTR5) has been reported to be expressed in adrenal, aorta, brain, breast, eye, heart, pancreas, prostate, skeletal muscle, small intestine, and thyroid. ESTs have been isolated from cartilage libraries.
G-protein Coupled Receptors (GPCRs) comprise one of the largest families of signaling molecules with more than a thousand members currently predicted to exist. All GPCRs share a structural motif consisting of seven membrane-spanning helices, and exist in both active and inactive forms. An array of activating ligands participate in the conformation of GPCRs which leads to signaling via G-proteins and downstream effectors. Ongoing studies have also shown the vast series of reactions which participate in the negative regulation of GPCRs. This "turn-off" activity has tremendous implications for the physiological action of the cell, and continues to drive pharmacological research for new drug candidates. Two blockbuster drugs which have been developed as GPCR-targeted pharmaceuticals are Zyprexa (Eli Lilly) and Claritin (Schering-Plough) which have multi-billion dollar shares of the mental health and allergy markets, respectively
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Somatostatin receptor subtypes in human pheochromocytoma: subcellular expression pattern and functional relevance for octreotide scintigraphy.
PA3-112 was used in immunohistochemistry to investigate the cellular distribution of five somatostatin receptor subtypes in human pheochromocytoma
|Mundschenk J,Unger N,Schulz S,Höllt V,Schulz S,Steinke R,Lehnert H||The Journal of clinical endocrinology and metabolism (88:5150)||2003|