Invitrogen

Synaptojanin 1 Monoclonal Antibody (AC1)

Product Image Gallery

Synaptojanin 1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of Synaptojanin 1 (green) showing staining in the cytoplasm of U-87 MG cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Synaptojanin 1 monoclonal antibody (Product # MA3-936) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Synaptojanin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Synaptojanin 1 showing staining in the cytoplasm of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Synaptojanin 1 Mouse Monoclonal Antibody (Product # MA3-936) diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Synaptojanin 1 Antibody in Western Blot (WB) — Western blot analysis of Synaptojanin 1 was performed by loading 25 µg of C6 cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a Synaptojanin 1 monoclonal antibody (Product # MA3-936) at a dilution of 1:500 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~170 kDa.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:10-1:100

Immunofluorescence (IF)

1:10-1:100

Immunohistochemistry (Frozen) (IHC (F))

1/20

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunoprecipitation (IP)

Assay Dependent

Western Blot (WB)

1:2000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Rat

Published species

Mouse

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

AC1

Immunogen

Proline rich domain of a fusion protein comprising amino acids 1156-1286 of rat synaptojanin 1.

Conjugate

Unconjugated

Form

Liquid

Storage buffer

ascites diluted in PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2201023

Product Specific Information

MA3-936 detects Synaptojanin 1 from rat and human samples.

MA3-936 has been successfully used in Western blotting, IHC (frozen), IP and ICC/IF procedures. By Western blot MA3-936 detects a band at 170 kDa representing Synaptojanin 1.

The MA3-936 immunogen is proline rich domain of a fusion protein comprising aa 1156-1286 of rat synaptojanin 1.

Target Information

Synaptojanin 1 is a phosphoinositide phosphatase that regulates levels of membrane phosphatidylinositol-4,5-bisphosphate. As such, expression of this enzyme may affect synaptic transmission and membrane trafficking.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: inositol 5'-phosphatase (synaptojanin 1); inositol polyphosphate-5-phosphatase G; phosphoinositide 5-phosphatase; Synaptic inositol 1,4,5-trisphosphate 5-phosphatase 1; synaptic inositol-1,4,5-trisphosphate 5-phosphatase 1; Synaptojanin-1; synaptojanin-1, polyphosphoinositide phosphatase

Gene Aliases: INPP5G; KIAA0910; PARK20; SYNJ1

UniProt ID: (Human) O43426, (Rat) Q62910

Entrez Gene ID: (Human) 8867, (Rat) 85238

Molecular Function: hydrolase phosphatase