Invitrogen

Synaptojanin 1 Monoclonal Antibody (AC1)

Product Image Gallery

Synaptojanin 1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Synaptojanin 1 (green) showing staining in the cytoplasm of U-87 MG cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Synaptojanin 1 monoclonal antibody (Product # MA3-936) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Synaptojanin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Synaptojanin 1 showing staining in the cytoplasm of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Synaptojanin 1 Mouse Monoclonal Antibody (Product # MA3-936) diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Synaptojanin 1 Antibody in Western Blot (WB) — Western blot analysis of Synaptojanin 1 was performed by loading 25 µg of C6 cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a Synaptojanin 1 monoclonal antibody (Product # MA3-936) at a dilution of 1:500 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~170 kDa.

Product Details

MA3-936

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100	-
Immunohistochemistry (Frozen) (IHC (F))	1/20	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Immunoprecipitation (IP)	Assay-dependent	-

Product Specifications
Species Reactivity	Human, Rat
Published species	Mouse
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	AC1
Immunogen	Proline rich domain of a fusion protein comprising amino acids 1156-1286 of rat synaptojanin 1. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	ascites diluted in PBS
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2201023

Product Specific Information

MA3-936 detects Synaptojanin 1 from rat and human samples.

MA3-936 has been successfully used in Western blotting, IHC (frozen), IP and ICC/IF procedures. By Western blot MA3-936 detects a band at 170 kDa representing Synaptojanin 1.

The MA3-936 immunogen is proline rich domain of a fusion protein comprising aa 1156-1286 of rat synaptojanin 1.

Target Information

Synaptojanin 1 is a phosphoinositide phosphatase that regulates levels of membrane phosphatidylinositol-4,5-bisphosphate. As such, expression of this enzyme may affect synaptic transmission and membrane trafficking.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Overexpression of endophilin A1 exacerbates synaptic alterations in a mouse model of Alzheimer's disease.

Nature communications

Yu Q,Wang Y,Du F,Yan S,Hu G,Origlia N,Rutigliano G,Sun Q,Yu H,Ainge J,Yan SF,Gunn-Moore F,Yan SS

MA3-936 was used in Western Blotting to study the in vivo consequences of the upregulation of Endophilin A1 expression in amyloid-Β peptide-rich environments.

Mon Jul 30 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: inositol 5'-phosphatase (synaptojanin 1); inositol polyphosphate-5-phosphatase G; phosphoinositide 5-phosphatase; Synaptic inositol 1,4,5-trisphosphate 5-phosphatase 1; synaptic inositol-1; synaptic inositol-1,4,5-trisphosphate 5-phosphatase 1; Synaptojanin-1; synaptojanin-1, polyphosphoinositide phosphatase

Gene Aliases: INPP5G; KIAA0910; PARK20; SYNJ1

UniProt ID: (Human) O43426, (Rat) Q62910

Entrez Gene ID: (Human) 8867, (Rat) 85238

Molecular Function: phosphatase