Immunofluorescence analysis of Synaptophysin was done on 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Synaptophysin (SP11) Rabbit Monoclonal Antibody (MA514532) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide of human synaptophysin|
|Storage buffer||tissue culture supernatant|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2-3 µg/mL|
|Immunofluorescence (IF)||2-3 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 3 publications below|
|Immunohistochemistry (IHC)||See 25 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
MA5-14532 targets Synaptophysin in IHC (P) and WB applications and shows reactivity with Human samples.
The MA5-14532 immunogen is a synthetic peptide of human synaptophysin.
This antibody recognizes a protein of 38kDa, identified as synaptophysin. It labels normal neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, gastrointestinal mucosa, Paneth's cells in the gastrointestinal tract and of gastric parietal cells. Neurons in the brain, spinal cord, and retina are also labeled. In combination with anti-chromogranin A and anti-NSE, Ab to synaptophysin is very useful in the identification of normal neuroendocrine cells and neuroendocrine neoplasms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Complement activation at the motor end-plates in amyotrophic lateral sclerosis.
MA5-14532 was used in immunohistochemistry - frozen section to analyze amyotrophic lateral sclerosis and complement activation at the motor-end plates
|Bahia El Idrissi N,Bosch S,Ramaglia V,Aronica E,Baas F,Troost D||Journal of neuroinflammation (13:null)||2016|
Bcl-xL promotes metastasis independent of its anti-apoptotic activity.
MA5-14532 was used in immunohistochemistry - paraffin section to show that Bcl-xL promotes metastasis independent of its anti-apoptotic activity
|Choi S,Chen Z,Tang LH,Fang Y,Shin SJ,Panarelli NC,Chen YT,Li Y,Jiang X,Du YC||Nature communications (7:null)||2016|
Complex APC germline mutation associated metaplasia and intraepithelial neoplasia (CAM-IEN) of the gallbladder.
MA5-14532 was used in immunohistochemistry - paraffin section to report a unique case of familial adenomatous polyposis
|Böger C,Haag J,Egberts JH,Röcken C||Pathology, research and practice (212:54)||2016|
Ewing sarcoma of the small bowel: a study of seven cases, including one with the uncommonly reported EWSR1-FEV translocation.
MA5-14532 was used in immunohistochemistry to discuss Ewing sarcoma.
|Milione M,Gasparini P,Sozzi G,Mazzaferro V,Ferrari A,Casali PG,Perrone F,Tamborini E,Pellegrinelli A,Gherardi G,Arrigoni G,Collini P,Testi A,De Paoli E,Aiello A,Pilotti S,Pelosi G||Histopathology (64:1014)||2014|
A case of prostatic adenocarcinoma with aberrant p63 expression: presentation with detailed immunohistochemical study and FISH analysis.
MA5-14532 was used in immunohistochemistry - paraffin section to report a new clinical case of prostatic adenomas with abnormal p63 expression
|Baydar DE,Kulac I,Gurel B,De Marzo A||International journal of surgical pathology (19:131)||2011|
Does the WHO 2010 classification of pancreatic neuroendocrine neoplasms accurately characterize pancreatic neuroendocrine carcinomas?
MA5-14532 was used in immunohistochemistry to determine if pancreatic neuroendocrine carcinomas are accurately characterized by the WHO 2010 classification of pancreatic neuroendocrine neoplasms
|Hijioka S,Hosoda W,Mizuno N,Hara K,Imaoka H,Bhatia V,Mekky MA,Tajika M,Tanaka T,Ishihara M,Yogi T,Tsutumi H,Fujiyoshi T,Sato T,Hieda N,Yoshida T,Okuno N,Shimizu Y,Yatabe Y,Niwa Y,Yamao K||Journal of gastroenterology (50:564)||2015|
Prognostic impact of diagnosing colorectal neuroendocrine carcinoma using the World Health Organization 2010 classification.
MA5-14532 was used in immunohistochemistry to study the diagnosis of colorectal neuroendocrine carcinoma using the WHO 2010 classification
|Lee JL,Yu CS,Kim M,Hong SM,Lim SB,Kim JC||Surgery (155:650)||2014|
Evaluation of Ki-67 index in EUS-FNA specimens for the assessment of malignancy risk in pancreatic neuroendocrine tumors.
MA5-14532 was used in immunohistochemistry to study the malignancy risk of pancreatic neuroendocrine tumors based on the Ki67 index of endoscopic ultrasound-guided fine-needle aspiration samples
|Hasegawa T,Yamao K,Hijioka S,Bhatia V,Mizuno N,Hara K,Imaoka H,Niwa Y,Tajika M,Kondo S,Tanaka T,Shimizu Y,Kinoshita T,Kohsaki T,Nishimori I,Iwasaki S,Saibara T,Hosoda W,Yatabe Y||Endoscopy (46:32)||2014|
Expression of aldo-keto reductase family 1 member C3 (AKR1C3) in neuroendocrine tumors and adenocarcinomas of pancreas, gastrointestinal tract, and lung.
MA5-14532 was used in immunohistochemistry to examine neuroendocrine tumors and a range of adenocarcinomas for the expression of the ketosteroid reducing enzyme AKR1C3
|Chang TS,Lin HK,Rogers KA,Brame LS,Yeh MM,Yang Q,Fung KM||International journal of clinical and experimental pathology (6:2419)||2013|
Concurrent AURKA and MYCN gene amplifications are harbingers of lethal treatment-related neuroendocrine prostate cancer.
MA5-14532 was used in immunohistochemistry to study the potential of Aurora kinase A and N-myc gene amplifications as predictive/prognostic biomarkers for the development of treatment-related neuroendocrine prostate cancer
|Mosquera JM,Beltran H,Park K,MacDonald TY,Robinson BD,Tagawa ST,Perner S,Bismar TA,Erbersdobler A,Dhir R,Nelson JB,Nanus DM,Rubin MA||Neoplasia (New York, N.Y.) (15:1)||2013|
Hyperplasia of pulmonary neuroepithelial bodies (NEB) in lungs of prolyl hydroxylase -1(PHD-1) deficient mice.
MA5-14532 was used in immunohistochemistry to study the effects of PHD-1 deficiency on neuroepithelial body hyperplasia in a murine model
|Pan J,Yeger H,Ratcliffe P,Bishop T,Cutz E||Advances in experimental medicine and biology (758:149)||2012|
Pigmented paraganglioma of the kidney: a case report.
MA5-14532 was used in immunohistochemistry to report on a case of pigmented paraganglioma of the kidney
|Zhao L,Luo J,Zhang H,Da J||Diagnostic pathology (7:null)||2012|
Is primary pulmonary meningioma a giant form of a meningothelial-like nodule? A case report and review of the literature.
MA5-14532 was used in immunohistochemistry to report on a patient with large pulmonary meningothelial-like nodules
|Masago K,Hosada W,Sasaki E,Murakami Y,Sugano M,Nagasaka T,Yamada M,Yatabe Y||Case reports in oncology (5:471)||2012|
Human achaete-scute homolog-1 expression in neuroendocrine breast carcinoma.
MA5-14532 was used in immunohistochemistry to study the expression of the hASH-1 transcription factor in neuroendocrine breast carcinoma
|Righi L,Rapa I,Votta A,Papotti M,Sapino A||Virchows Archiv : an international journal of pathology (460:415)||2012|
Acinar cell carcinoma: a possible diagnosis in patients without intrapancreatic tumour.
MA5-14532 was used in immunohistochemistry to report three clinical cases of acinar cell carcinoma without intrapancreatic tumour
|Terris B,Genevay M,Rouquette A,Audebourg A,Mentha G,Dousset B,Rubbia-Brandt L||Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver (43:971)||2011|
Expression of p63 is the sole independent marker of aggressiveness in localised (stage I-II) Merkel cell carcinomas.
MA5-14532 was used in immunohistochemistry to study the prognostic value of p63 expression in Merkel cell carcinomas
|Asioli S,Righi A,de Biase D,Morandi L,Caliendo V,Picciotto F,Macripò G,Maletta F,di Cantogno LV,Chiusa L,Eusebi V,Bussolati G||Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (24:1451)||2011|
Tumor-in-tumor of the thyroid with basaloid differentiation: a lesion with a solid cell nest neoplastic component?
MA5-14532 was used in immunohistochemistry to report on a case of tumor-in-tumor of the thyroid with basaloid differentiation
|Eloy C,Vinagre J,Cameselle-Teijeiro J,Paiva ME,Soares P,Sobrinho-Simões M||International journal of surgical pathology (19:276)||2011|
Detection of bone marrow metastases of neuroblastoma with immunohistochemical staining of CD56, chromogranin A, and synaptophysin.
MA5-14532 was used in immunohistochemistry to detect the bone marrow metastases of neuroblastoma by immunohistochemical staining of specific proteins
|Park SJ,Park CJ,Kim S,Jang S,Chi HS,Kim MJ,Im HJ,Seo JJ||Applied immunohistochemistry and molecular morphology : AIMM (18:348)||2010|
Cerebellar abiotrophy in an alpaca (Lama pacos).
MA5-14532 was used in immunohistochemistry to characterize cerebellar abiotrophy in an alpaca
|Mouser P,Lévy M,Sojka JE,Ramos-Vara JA||Veterinary pathology (46:1133)||2009|
Small cell carcinoma with skeletal muscle differentiation of urinary bladder.
MA5-14532 was used in immunohistochemistry to report a clinical case of small cell carcinoma with skeletal muscle differentiation of urinary bladder
|Yajima N,Mizukami H,Wada R,Saito F,Yagihashi S||Pathology international (59:748)||2009|
Identification of carcinoma origin by thyroid transcription factor-1 immunostaining of fine needle aspirates of metastases.
MA5-14532 was used in immunohistochemistry to study the identification of carcinoma origin using thyroid transcription factor-1 immunostaining of metastases
|Strojan Flezar M,Srebotnik Kirbis I||Cytopathology : official journal of the British Society for Clinical Cytology (20:176)||2009|
Confusing cases: clear cell but not renal cell lesions in kidney.
MA5-14532 was used in immunohistochemistry to examine the diagnostic process for clear cell and renal cell lesions in kidney
|Baydar D,Aydin O||Pathology international (58:713)||2008|
Atypical cystic meningioma overexpressing AQP1 in early infancy: case report with literature review.
MA5-14532 was used in immunohistochemistry to examine a case of neonatal atypical cystic meningioma
|Marton E,Feletti A,Basaldella L,Dei Tos AP,Bendini M,Longatti P||Acta paediatrica (Oslo, Norway : 1992) (97:1145)||2008|
Endolymphatic sac tumor (aggressive papillary tumor of middle ear and temporal bone): report of two cases with analysis of the VHL gene.
MA5-14532 was used in immunohistochemistry to report on two cases of endolymphatic sac tumor
|Skalova A,Síma R,Bohus P,Curík R,Lukás J,Michal M||Pathology, research and practice (204:599)||2008|
Reactivity with TdT in Merkel cell carcinoma: a potential diagnostic pitfall.
MA5-14532 was used in immunohistochemistry to study potential lymphoma diagnostic issues due to immunohistochemical reactivity of TdT in Merkel cell carcinoma
|Buresh CJ,Oliai BR,Miller RT||American journal of clinical pathology (129:894)||2008|
Diagnosis of biliary tract lesions by histological sectioning of brush bristles as alternative to cytological smearing.
MA5-14532 was used in immunohistochemistry to study histological sectioning of brush bristles as an alternative method for diagnosis of biliary tract lesions
|Asioli S,Accinelli G,Pacchioni D,Bussolati G||The American journal of gastroenterology (103:1274)||2008|
A novel assessment of the quality of immunohistostaining overcomes the limitations of current methods.
MA5-14532 was used in immunohistochemistry to establish a straightforward procedure for objective quality evaluation of the components involved in immunohistostaining
|Eisenthal A,Trejo L,Shtabsky A,Bedny F,Brazowski E||Pathology, research and practice (204:323)||2008|
p63 expression as a new prognostic marker in Merkel cell carcinoma.
MA5-14532 was used in immunohistochemistry to study p63 expression as a prognostic marker in Merkel cell carcinoma
|Asioli S,Righi A,Volante M,Eusebi V,Bussolati G||Cancer (110:640)||2007|
The contribution of bifunctional SkipDewax pretreatment solution, rabbit monoclonal antibodies, and polymer detection systems in immunohistochemistry.
MA5-14532 was used in immunohistochemistry to evaluate novel immunohistochemistry reagents and techniques
|Wong SC,Chan JK,Lo ES,Chan AK,Wong MC,Chan CM,Lam MY,Chan AT||Archives of pathology and laboratory medicine (131:1047)||2007|
Clinico-pathological features of a series of 11 oncocytic endocrine tumours of the pancreas.
MA5-14532 was used in immunohistochemistry to investigate the characteristic of the oncocytic endocrine tumours of the pancreas
|Volante M,La Rosa S,Castellano I,Finzi G,Capella C,Bussolati G||Virchows Archiv : an international journal of pathology (448:545)||2006|
Pulmonary neuroendocrine cells, airway innervation, and smooth muscle are altered in Cftr null mice.
MA5-14532 was used in immunocytochemistry to study the effect of Cftr knockout on the number and distribution of pulmonary neuroendocrine cells and on airway smooth muscle ennervation
|Pan J,Luk C,Kent G,Cutz E,Yeger H||American journal of respiratory cell and molecular biology (35:320)||2006|