Immunohistochemical staining of paraformaldehyde-fixed paraffin-embedded human cerebellum sections at 200x (A) and 400x (B) magnification with DAPI counterstain. Primary antibody used was a Syntaxin-1 monoclonal antibody (clone SP6) at a 1:500 dilution, followed by secondary antibody was a 488 fluorescent conjugated Goat anti-mouse Ig at a 1:500 dilution and Normal Goat Serum (blocking)
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||0.5 ug/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17612 targets Syntaxin-1 in ELISA, FACS, and WB applications and shows reactivity with Human, Mouse, and Rat samples.
The MA5-17612 immunogen is human Syntaxin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Histone deacetylase expression in white matter oligodendrocytes after stroke.
MA5-17612 was used in immunohistochemistry to measure the expression of individual HDACs in the oligodendrocyte lineage during stroke-induced oligodendrogenesis
|Kassis H,Chopp M,Liu XS,Shehadah A,Roberts C,Zhang ZG||Neurochemistry international (77:17)||2014|
Intrahepatic myeloid-cell aggregates enable local proliferation of CD8(+) T cells and successful immunotherapy against chronic viral liver infection.
MA5-17612 was used in immunohistochemistry to study the local expansion of CD8-positice T-cells in intrahepatic myeloid-cell aggregates and the implications for chronic hepatic viral infection immunotherapy
|Huang LR,Wohlleber D,Reisinger F,Jenne CN,Cheng RL,Abdullah Z,Schildberg FA,Odenthal M,Dienes HP,van Rooijen N,Schmitt E,Garbi N,Croft M,Kurts C,Kubes P,Protzer U,Heikenwalder M,Knolle PA||Nature immunology (14:574)||2013|
Compton scattering by internal shields based on melanin-containing mushrooms provides protection of gastrointestinal tract from ionizing radiation.
MA5-17612 was used in immunohistochemistry to study the ability of oral administration of mushrooms containing melanin to protect the gut against ionizing radiation
|Revskaya E,Chu P,Howell RC,Schweitzer AD,Bryan RA,Harris M,Gerfen G,Jiang Z,Jandl T,Kim K,Ting LM,Sellers RS,Dadachova E,Casadevall A||Cancer biotherapy and radiopharmaceuticals (27:570)||2012|
DNA damage signaling regulates age-dependent proliferative capacity of quiescent inner ear supporting cells.
MA5-17612 was used in immunohistochemistry - paraffin section to study how proliferative restrictions of supporting cells are linked with DNA damage signaling
|Laos M,Anttonen T,Kirjavainen A,af Hällström T,Laiho M,Pirvola U||Aging (6:496)||2014|