|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG|
|Immunogen||Recombinant protein corresponding to aa 102-269 of human TCF1A splice variant protein fused to maltose binding protein (MBP)|
|Storage buffer||0.1M tris glycine, pH 7.4, with 30% glycerol, 0.15M NaCl|
|Contains||0.08% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Western Blot (WB)||0.5 - 2 µg/ml|
This antibody recognizes multiple splice isoforms of TCF-1.
TCF 1 (T cell factor 1) is a member of the TCF/LEF (lymphoid enhancer factor) family of transcription factors. Members of this family along with b catenin, are involved in mediating Wnt signaling by transactivating downstream genes. TCF 1 is closely related to LEF-1, both of which are transcription factors expressed in mice during T cell differentiation. They regulate the T cell receptor a enhancer in transfections assays. TCF 1 becomes transcriptionally active through interaction with b catenin. Also, numerous splice variants of TCF 1 have been identified. Overexpression of TCF-1-specific splice forms appears to correlate with the metastatic behavior of colorectal cancer cells and with overproduction of lymphoid tyrosine protein kinase p56(lck).