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Flow cytometry analysis of TCR delta on TCR delta positive PEER cells (left panel) or TCR delta negative HPB-ALL cells (right panel). Equal numbers of cells were stained with a TCR Delta TCS1 (TS-1) monoclonal antibody (Product # TCR1055) followed by FITC labeled secondary antibody, or FITC labeled secondary antibody alone (black histograms). 5ul of primary antibody were used per test. All antibody incubations were performed for 30 minutes at room temperature. A representative 10,000 cells were acquired for each sample.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human TCR Vdelta1-Jdelta1|
|Storage buffer||PBS with 0.5% BSA, glycerol|
|Contains||0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-2 µg/test|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|T-Cell Activation (TCA)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
TCR1055 targets TCR Delta TCS1 in FACS, IHC(F), IP, and TCA applications and shows reactivity with Human samples.
The TCR1055 immunogen is human TCR Vdelta1-Jdelta1.
TCR1055 detects TCR Delta TCS1 which has a predicted molecular weight of approximately 17 kDa.
The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5). T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Human cytomegalovirus elicits fetal gammadelta T cell responses in utero.
TCR1055 was used in flow cytometry to examine the effect of human cytomegalovirus on fetal gammadelta T cell responses in utero
|Vermijlen D,Brouwer M,Donner C,Liesnard C,Tackoen M,Van Rysselberge M,Twité N,Goldman M,Marchant A,Willems F||The Journal of experimental medicine (207:807)||2010|
Human CD1-restricted T cell recognition of lipids from pollens.
TCR1055 was used in flow cytometry to study the immune response of pollen lipids
|Agea E,Russano A,Bistoni O,Mannucci R,Nicoletti I,Corazzi L,Postle AD,De Libero G,Porcelli SA,Spinozzi F||The Journal of experimental medicine (202:295)||2005|
Skewed inhibitory receptors expression in a TAP2-deficient patient.
TCR1055 was used in flow cytometry to study the expression of IRs in TAP-deficient patients
|Crespí C,Rosa Julià M,Muñoz-Saa I,Pérez-Castellano MT,Milà J,Matamoros N||Immunology letters (86:149)||2003|
Perturbation and proinflammatory type activation of V delta 1(+) gamma delta T cells in African children with Plasmodium falciparum malaria.
TCR1055 was used in flow cytometry to study the expanded population of Vdelta1+ T cells in Plasmodium falciparum malaria patients
|Hviid L,Kurtzhals JA,Adabayeri V,Loizon S,Kemp K,Goka BQ,Lim A,Mercereau-Puijalon O,Akanmori BD,Behr C||Infection and immunity (69:3190)||2001|
New monoclonal antibody (23D12) recognizing three different V gamma elements of the human gamma delta T cell receptor. 23D12+ cells comprise a major subpopulation of gamma delta T cells in postnatal thymus.
TCR1055 was used in flow cytometry to characterize a monoclonal antibody against human gamma delta T cell receptor
|Kabelitz D,Ackermann T,Hinz T,Davodeau F,Band H,Bonneville M,Janssen O,Arden B,Schondelmaier S||Journal of immunology (Baltimore, Md. : 1950) (152:3128)||1994|
Recognition by human V gamma 9/V delta 2 T cells of a GroEL homolog on Daudi Burkitt's lymphoma cells.
TCR1055 was used in flow cytometry to investigate the interaction between human gamma delta T cells expressing TCR V gamma 9, V delta 2, and groEL homolog on Daudi cells
|Fisch P,Malkovsky M,Kovats S,Sturm E,Braakman E,Klein BS,Voss SD,Morrissey LW,DeMars R,Welch WJ||Science (New York, N.Y.) (250:1269)||1990|
Human cytotoxic lymphocytes. V. Frequency and specificity of gamma delta+ cytotoxic lymphocyte precursors activated by allogeneic or autologous stimulator cells.
TCR1055 was used in flow cytometry to characterize gamma delta+ cytotoxic lymphocyte precursors and its activation mechanism
|Kabelitz D,Bender A,Schondelmaier S,da Silva Lobo ML,Janssen O||Journal of immunology (Baltimore, Md. : 1950) (145:2827)||1990|
Gastrointestinal T cell lymphoma: predominant cytotoxic phenotypes, including alpha/beta, gamma/delta T cell and natural killer cells.
TCR1055 was used in immunohistochemistry to investigate the cytotoxic phenotypes of gastrointestinal T cell lymphomas
|Katoh A,Ohshima K,Kanda M,Haraoka S,Sugihara M,Suzumiya J,Kawasaki C,Shimazaki K,Ikeda S,Kikuchi M||Leukemia & lymphoma (39:97)||2000|
Immunological study on CD3 defective cutaneous T cell lymphoma cells from a patient with Sézary syndrome.
TCR1055 was used in immunohistochemistry to study the effect of CD3 surface expression on cutaneous T cell lymphoma cells
|Sano S,Matsui Y,Itami S,Yoshikawa K||Clinical and experimental immunology (113:190)||1998|
Monoclonal antibodies to T cell receptor gamma/delta complex react with human endometrial glandular epithelium.
TCR1055 was used in immunohistochemistry to study the expression of T cell receptor gamma/delta complex in endometrial glandular epithelium
|Yeh CJ,Bulmer JN,Hsi BL,Tian WT,Rittershaus C,Ip SH||Placenta (11:253)||1990|