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Western blot analysis of TCR V gamma 9 was performed by loading 25 ug of CEM cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a TCR V gamma 9 monoclonal antibody (Product # TCR1720) at a dilution of 1:10 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~18-28 kDa.
|Tested species reactivity||Human, Non-human primate|
|Published species reactivity||Non-human primate, Fruit fly, Human, Rhesus monkey|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human TCR Vgamma9|
|Storage buffer||PBS with 0.5% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||2 ug/test|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Neutralization (Neu)||Assay Dependent|
|T-Cell Activation (TCA)||Assay Dependent|
|Western Blot (WB)||1:1-1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
|Blocking Assay (BLOCK)||See 1 publications below|
|Flow Cytometry (Flow)||See 11 publications below|
|ELISA (ELISA)||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
|Immunocytochemistry (ICC)||See 2 publications below|
TCR1720 targets TCR V gamma 9 in FACS, IHC(F), IP, Neu, WB and TCA applications and shows reactivity with Human, and Non-human primate samples.
The TCR1720 immunogen is human TCR Vgamma9.
The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5). T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Phosphoantigen/IL2 expansion and differentiation of Vγ2Vδ2 T cells increase resistance to tuberculosis in nonhuman primates.
TCR1720 was used in immunohistochemistry to study the increased Tuberculosis resistance of non-human primates following phoshoantigen/IL-2 mediated expansion and differentiation of VgammaVdelta2 T-cells
|Chen CY,Yao S,Huang D,Wei H,Sicard H,Zeng G,Jomaa H,Larsen MH,Jacobs WR,Wang R,Letvin N,Shen Y,Qiu L,Shen L,Chen ZW||PLoS pathogens (9:null)||2013|
Mechanisms of the antitumor activity of human Vγ9Vδ2 T cells in combination with zoledronic acid in a preclinical model of neuroblastoma.
TCR1720 was used in blocking or activating experiment to study the mechanisms underlying the therapautic activity of T-cell receptor Vgamma9Vdelta2 lymphocytes and zoledronic acid in a neuroblastoma model
|Di Carlo E,Bocca P,Emionite L,Cilli M,Cipollone G,Morandi F,Raffaghello L,Pistoia V,Prigione I||Molecular therapy : the journal of the American Society of Gene Therapy (21:1034)||2013|
|Rhesus monkey||Not Cited||
Multieffector-functional immune responses of HMBPP-specific Vγ2Vδ2 T cells in nonhuman primates inoculated with Listeria monocytogenes ΔactA prfA*.
TCR1720 was used in flow cytometry to study multi-effector functional Vgamma2Vdelta2 T cell responses against Listeria monocytogenes in nonhuman primates
|Ryan-Payseur B,Frencher J,Shen L,Chen CY,Huang D,Chen ZW||Journal of immunology (Baltimore, Md. : 1950) (189:1285)||2012|
|Non-human primate||Not Cited||
Differentiation, distribution and gammadelta T cell-driven regulation of IL-22-producing T cells in tuberculosis.
TCR1720 was used in flow cytometry to characterize interleukin 12 producing T cells in tuberculosis
|Yao S,Huang D,Chen CY,Halliday L,Zeng G,Wang RC,Chen ZW||PLoS pathogens (6:null)||2010|
|Non-human primate||Not Cited||
Phosphoantigen-activated V gamma 2V delta 2 T cells antagonize IL-2-induced CD4+CD25+Foxp3+ T regulatory cells in mycobacterial infection.
TCR1720 was used in flow cytometry to investigate the effect of phosphoantigen-activated V gamma 2V delta 2 T cells for interleukin-2-induced CD4+CD25+Foxp3+ T regulatory cells in mycobacterial infection
|Gong G,Shao L,Wang Y,Chen CY,Huang D,Yao S,Zhan X,Sicard H,Wang R,Chen ZW||Blood (113:837)||2009|
Human CD1-restricted T cell recognition of lipids from pollens.
TCR1720 was used in flow cytometry to study the immune response of pollen lipids
|Agea E,Russano A,Bistoni O,Mannucci R,Nicoletti I,Corazzi L,Postle AD,De Libero G,Porcelli SA,Spinozzi F||The Journal of experimental medicine (202:295)||2005|
Recognition of nonpeptide antigens by human V gamma 9V delta 2 T cells requires contact with cells of human origin.
TCR1720 was used in flow cytometry to study the mechanism for nonpeptide antigen recognition by human Vgamma9Vdelta2 T cells
|Green AE,Lissina A,Hutchinson SL,Hewitt RE,Temple B,James D,Boulter JM,Price DA,Sewell AK||Clinical and experimental immunology (136:472)||2004|
|Non-human primate||Not Cited||
Inhibition of adaptive Vgamma2Vdelta2+ T-cell responses during active mycobacterial coinfection of simian immunodeficiency virus SIVmac-infected monkeys.
TCR1720 was used in flow cytometry to investigate the effect of mycobacterial coinfection of simian immunodeficiency virus on adaptive Vgamma2Vdelta2+ T-cell responses
|Zhou D,Lai X,Shen Y,Sehgal P,Shen L,Simon M,Qiu L,Huang D,Du GZ,Wang Q,Letvin NL,Chen ZW||Journal of virology (77:2998)||2003|
Perturbation and proinflammatory type activation of V delta 1(+) gamma delta T cells in African children with Plasmodium falciparum malaria.
TCR1720 was used in flow cytometry to study the expanded population of Vdelta1+ T-cell in P. falciparum malaria patients
|Hviid L,Kurtzhals JA,Adabayeri V,Loizon S,Kemp K,Goka BQ,Lim A,Mercereau-Puijalon O,Akanmori BD,Behr C||Infection and immunity (69:3190)||2001|
Cytotoxic hepatosplenic gammadelta T-cell lymphoma following acute myeloid leukemia bearing two distinct gamma chains of the T-cell receptor. Biologic and clinical features.
TCR1720 was used in flow cytometry to study the expression of Vd1 and two distinct TCRg chains, Vg3 and Vg9 during hepatosplenic gd-T-cell lymphoma
|Weidmann E,Hinz T,Klein S,Schui DK,Harder S,Kriener S,Kabelitz D,Hoelzer D,Mitrou PS||Haematologica (85:1024)||2000|
Involvement of the Fas/Fas ligand pathway in activation-induced cell death of mycobacteria-reactive human gamma delta T cells: a mechanism for the loss of gamma delta T cells in patients with pulmonary tuberculosis.
TCR1720 was used in flow cytometry to study human gamma delta T cells in pulmonary tuberculosis patients
|Li B,Bassiri H,Rossman MD,Kramer P,Eyuboglu AF,Torres M,Sada E,Imir T,Carding SR||Journal of immunology (Baltimore, Md. : 1950) (161:1558)||1998|
New monoclonal antibody (23D12) recognizing three different V gamma elements of the human gamma delta T cell receptor. 23D12+ cells comprise a major subpopulation of gamma delta T cells in postnatal thymus.
TCR1720 was used in flow cytometry to characterize a monoclonal antibody against human gamma delta T cell receptor
|Kabelitz D,Ackermann T,Hinz T,Davodeau F,Band H,Bonneville M,Janssen O,Arden B,Schondelmaier S||Journal of immunology (Baltimore, Md. : 1950) (152:3128)||1994|
Human cytotoxic lymphocytes. V. Frequency and specificity of gamma delta+ cytotoxic lymphocyte precursors activated by allogeneic or autologous stimulator cells.
TCR1720 was used in flow cytometry to characterize human cytotoxic lymphocytes
|Kabelitz D,Bender A,Schondelmaier S,da Silva Lobo ML,Janssen O||Journal of immunology (Baltimore, Md. : 1950) (145:2827)||1990|
Reciprocal interactions between human mesenchymal stem cells and gammadelta T cells or invariant natural killer T cells.
TCR1720 was used in ELISA to investigate the interactions between mesenchymal stem cells and gammadelta T cells
|Prigione I,Benvenuto F,Bocca P,Battistini L,Uccelli A,Pistoia V||Stem cells (Dayton, Ohio) (27:693)||2009|
|Fruit fly||Not Cited||
Definition of APC presentation of phosphoantigen (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate to Vgamma2Vdelta 2 TCR.
TCR1720 was used in western blot to study the antigen presentation of a specific phosphoantigen
|Wei H,Huang D,Lai X,Chen M,Zhong W,Wang R,Chen ZW||Journal of immunology (Baltimore, Md. : 1950) (181:4798)||2008|
The bisphosphonate acute phase response: rapid and copious production of proinflammatory cytokines by peripheral blood gd T cells in response to aminobisphosphonates is inhibited by statins.
TCR1720 was used in immunocytochemistry to characterize the acute phase response by bisphosphonates
|Hewitt RE,Lissina A,Green AE,Slay ES,Price DA,Sewell AK||Clinical and experimental immunology (139:101)||2005|
Polymyositis mediated by T lymphocytes that express the gamma/delta receptor.
TCR1720 was used in immunocytochemistry to investigate the role of TCR gamma/delta T cells in polymyositis
|Hohlfeld R,Engel AG,Ii K,Harper MC||The New England journal of medicine (324:877)||1991|