Invitrogen

TDG Monoclonal Antibody (GT1511)

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TDG Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of TDG was performed in paraffin-embedded rat intestine tissue using TDG Monoclonal Antibody (GT1511) (Product # MA5-31522) at a dilution of 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

TDG Antibody in Western Blot (WB) — Western blot analysis of TDG in 293T mock transfected (-) or transfected with a construct expressing human TDG using TDG monoclonal antibody (Product # MA5-31522) at a dilution of 1:10000. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:20,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

GT1511

Immunogen

Recombinant protein encompassing a sequence within the center region of human TDG.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2787153

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Human (99%), Mouse (88%), Rat (87%), Bovine (91%).

Positive Control: human TDG-transfected 293T cells.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mis paired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.