Invitrogen

TDP-43 Monoclonal Antibody (GT733)

TDP-43 Antibody in Immunocytochemistry (ICC/IF)

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TDP-43 Antibody (MA5-27828) in ICC/IF

Immunofluorescence of TDP-43 was performed using HAP1 wild-type and TARDBP KO cells that were transfected with a green or a far-red fluorescent dye, respectively. Post-transfection, WT and KO cells were mixed and plated to a 1:1 ratio on coverslips as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) or methanol for 15 min; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS with 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min. Cells were stained with the TDP-43 monoclonal ant... View More

TDP-43 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TDP-43 Antibody in Immunoprecipitation (IP)

TDP-43 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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Product Details

MA5-27828

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

GT733

Immunogen

Recombinant protein encompassing a sequence within the center region of human TDP43. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2735390

Product Specific Information

Positive Control: A431, HeLa, HepG2, 293T, TARDBP shRNA-transfected 293T, U87-MG, SK-N-SH, IMR32, SK-N-AS

Predicted Reactivity: Zebrafish (83%), Xenopus laevis (91%), Chicken (95%), Bovine (96%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

TDP43 was first identified as a novel cellular protein that binds to HIV-1 virus TAR DNA sequence motifs and acts a transcriptional repressor to the HIV-1 LTR. Later experiments revealed that TDP43 also regulates the splicing of exon 9 of the cystic fibrosis transmembrane conductance regular (CFTR), most likely through the association with the UG repeats at the 3"e; splice site. A hyperphosphorylated, ubiquitinated, and cleaved form of TDP43 known as pathologic TDP43 is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FLTD-U). TDP43 is not related to TRBP1, and RNA binding protein that binds HIV-1 TAR RNA sequences.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The identification of high-performing antibodies for TDP-43 for use in Western Blot, immunoprecipitation and immunofluorescence.

F1000Research

Worrall D,Ayoubi R,Fotouhi M,Southern K,McPherson PS,Laflamme C

Published figure using TDP-43 monoclonal antibody (Product # MA5-27828) in Western Blot

Wed Jun 28 00:00:00 UTC 2023

The identification of high-performing antibodies for TDP-43 for use in Western Blot, immunoprecipitation and immunofluorescence.

F1000Research

Worrall D,Ayoubi R,Fotouhi M,Southern K,McPherson PS,Laflamme C

Published figure using TDP-43 monoclonal antibody (Product # MA5-27828) in Western Blot

Wed Jun 28 00:00:00 UTC 2023

The identification of high-performing antibodies for TDP-43 for use in Western Blot, immunoprecipitation and immunofluorescence.

F1000Research

Worrall D,Ayoubi R,Fotouhi M,Southern K,McPherson PS,Laflamme C

Published figure using TDP-43 monoclonal antibody (Product # MA5-27828) in Western Blot

Wed Jun 28 00:00:00 UTC 2023

The identification of high-performing antibodies for TDP-43 for use in Western Blot, immunoprecipitation and immunofluorescence.

F1000Research

Worrall D,Ayoubi R,Fotouhi M,Southern K,McPherson PS,Laflamme C

Published figure using TDP-43 monoclonal antibody (Product # MA5-27828) in Western Blot

Wed Jun 28 00:00:00 UTC 2023

Bioinformatics

Protein Aliases: OTTHUMP00000002171; OTTHUMP00000002173; RP4-635E18.2; TAR DNA-binding protein 43; TAR DNA-binding protein-43; TDP-43

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Gene Aliases: 1190002A23Rik; ALS10; C85084; TARDBP; TDP-43; TDP43

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UniProt ID: (Human) Q13148, (Mouse) Q921F2

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Entrez Gene ID: (Human) 23435, (Mouse) 230908, (Rat) 298648

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Function(s)

nucleotide binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding RNA binding mRNA 3'-UTR binding protein binding identical protein binding poly(A) RNA binding nucleic acid binding DNA binding

Process(es)

negative regulation of protein phosphorylation transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter mRNA processing RNA splicing negative regulation of gene expression nuclear fragmentation involved in apoptotic nuclear change negative regulation by host of viral transcription positive regulation of transcription from RNA polymerase II promoter regulation of cell cycle 3'-UTR-mediated mRNA stabilization nuclear inner membrane organization positive regulation of insulin secretion response to endoplasmic reticulum stress

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TDP-43 Monoclonal Antibody (GT733)

TDP-43 Antibody (MA5-27828) in ICC/IF

Product Details

MA5-27828

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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