Invitrogen

TDP-43 Monoclonal Antibody (GT733)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (38) TDP-43 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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TDP-43 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of TDP43 in HeLa cells. Samples were treated with 4% paraformaldehyde at RT for 15 min and incubated with TDP43 monoclonal antibody (Product # MA5-27828) using a dilution of 1:500, followed by Hoechst.

TDP-43 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of TDP-43 in paraffin-embedded mouse brain. Sample was incubated with TDP-43 monoclonal antibody (Product # MA5-27828) using a dilution of 1:200.

TDP-43 Antibody in Western Blot (WB) — Western blot was performed using Anti-TDP-43 Monoclonal Antibody (GT733)(Product # MA5-27828) and a 44kDa band corresponding to TDP-43 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1) and K-562 (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

TDP-43 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated genome editing of target protein. A reduced signal was observed for the target protein in HeLa CAS9 cells transduced with TARDBP Lentiviral sgRNA compared to CAS9 control cell line using Anti-TDP-43 Monoclonal Antibody (GT733)(Product # MA5-27828) {KO}

Product Details

MA5-27828

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	GT733
Immunogen	Recombinant protein encompassing a sequence within the center region of human TDP43. The exact sequence is proprietary.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS
Contains	no preservative
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2735390

Product Specific Information

Positive Control: A431, HeLa, HepG2, 293T, TARDBP shRNA-transfected 293T, U87-MG, SK-N-SH, IMR32, SK-N-AS

Predicted Reactivity: Zebrafish (83%), Xenopus laevis (91%), Chicken (95%), Bovine (96%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

TDP43 was first identified as a novel cellular protein that binds to HIV-1 virus TAR DNA sequence motifs and acts a transcriptional repressor to the HIV-1 LTR. Later experiments revealed that TDP43 also regulates the splicing of exon 9 of the cystic fibrosis transmembrane conductance regular (CFTR), most likely through the association with the UG repeats at the 3"e; splice site. A hyperphosphorylated, ubiquitinated, and cleaved form of TDP43 known as pathologic TDP43 is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FLTD-U). TDP43 is not related to TRBP1, and RNA binding protein that binds HIV-1 TAR RNA sequences.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.