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Immunoprecipitation analysis using Anti-TDRD3 Recombinant Mouse Monoclonal Antibody (Cat. no. 730004) followed by Western Blot detection. Immunoprecipitation was performed on HEK293 cells over expressing flag-tagged TDRD3 protein. Western blot using an anti-flag antibody was performed on the raw lysate (lane 1) and immunoprecipitated protein (lane 2) to evaluate the results of Immunoprecipitation.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG|
|Immunogen||554-616 of 651 of TDRD3 Protein.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2 ug|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
TDRD3 belongs to the tudor domain group of proteins, which are prevalent among proteins involved in RNA metabolism. TDRD3 is widely expressed and localizes predominantly in the cytoplasm. It is detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Overexpression of TDRD3 in cells induces the formation of stress granules. Multiple isoforms of TDRD3 exist due to alternative splicing varients. In humans, the gene is located on chromosome 13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation.
730004 was used in immunoprecipitation to describe a mass spectrometry-based method for scoring immunoprecipitation antibody quality
|Marcon E,Jain H,Bhattacharya A,Guo H,Phanse S,Pu S,Byram G,Collins BC,Dowdell E,Fenner M,Guo X,Hutchinson A,Kennedy JJ,Krastins B,Larsen B,Lin ZY,Lopez MF,Loppnau P,Miersch S,Nguyen T,Olsen JB,Paduch M,Ravichandran M,Seitova A,Vadali G,Vogelsang MS,Whiteaker JR,Zhong G,Zhong N,Zhao L,Aebersold R,Arrowsmith CH,Emili A,Frappier L,Gingras AC,Gstaiger M,Paulovich AG,Koide S,Kossiakoff AA,Sidhu SS,Wodak SJ,Gräslund S,Greenblatt JF,Edwards AM||Nature methods (12:725)||2015|