|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant, full length human protein expressed in CHO cells.|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||2 - 4 µg/ml|
|Inhibition Assays (IA)||0.3 - 1 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
|Blocking Assay (BLOCK)||See 2 publications below|
MA1-24734 detects TGF beta 1 from human samples.
MA1-24734 has been successfully used in inhibition assay, ELISA, and Western blot procedures. By ELISA, the antibody shows less than 5% cross-reactivity with TGF-beta 1.2 and less than 1% cross reactivity with TGF beta 2, TGF beta 3, and TGF beta 5.
The MA1-24734 immunogen is recombinant, full length human protein expressed in CHO cells.
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Transforming Growth Factor Beta 1 is a multifunctional peptide capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed as well as non-neoplastic tissues, release transforming growth factors; and essentially all cells possess a specific TGF beta 1 receptor. The multi-modal nature of TGF beta 1 is seen in its ability to stimulate or inhibit cellular proliferation. In general, cells of mesenchymal origin appear to be stimulated by TGF beta 1 whereas hepatocytes, T and B lymphocytes, keratinocytes and many epithelial cells are inhibited by the peptide. TGF beta 1 interacts with Epidermal Growth Factor, Platelet Derived Growth Factor, Fibroblast Growth Factor, and IL2 either by enhancing or antagonizing their characteristic actions. TGF beta 1 plays a fundamental role in tissue growth and differentiation involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation and immune cell function.
Ectopic expression of homeobox gene NKX2-1 in diffuse large B-cell lymphoma is mediated by aberrant chromatin modifications.
MA1-24734 was used in blocking or activating experiment to study the role of aberrant chromatin modifications in driving the ectopic expression of NKX2-1 in diffuse large B-cell lymphoma
|Nagel S,Ehrentraut S,Tomasch J,Quentmeier H,Meyer C,Kaufmann M,Drexler HG,MacLeod RA||PloS one (8:null)||2013|
Residual methylprednisolone suppresses human T-cell responses to spleen, but not islet, extracts from deceased organ donors.
MA1-24734 was used in blocking or activating experiment to measure allogenic and auto-immune responses of human T cells in patients receiving pancreatic islet transplants
|Joffe M,Necula AS,Chand R,McWhinney BC,Krishnamurthy B,Loudovaris T,Goodman D,Thomas HE,Kay TW,Mannering SI||International immunology (24:447)||2012|