Immunofluorescent stainging of frozen mouse eye tissue sections with TLR3/CD283 polyclonal antibody (Product # PA5-23105), a retinal pigment epithelium (RPE) marker and DAPI. Top panel: TLR3 (red) colocalized with the RPE (green) marker. Bottom panel: tissue stained with isotype control (no TLR3 Ab), RPE and DAPI.
|Tested species reactivity||Human, Mouse, Pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A mix of synthetic peptides corresponding to amino acids 135-150, 828-844, and 876-891 of TLR3/CD283 protein.|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 µg/10^6 cells|
|Immunohistochemistry (Frozen) (IHC (F))||1:10-1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: mouse spleen lysate or protein.
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1) and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described (3). mTLR3 cDNA codes for a protein with approximate molecular weight of 100 kDa . TLR3 has a restricted expression pattern being expressed in dendritic cells (DC) (4-5). TLR3 mRNA expression was detected by in situ hybridization in DC and lymph nodes. The expression of TLR3 in a single cell type may indicate a specific role for this molecule in a restricted setting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.