Cell surface flow cytometric analysis of TLR4 in stable HEK293/hTLR4 cells using a TLR4/CD284 monoclonal antibody (Product # MA5-16205) at 1 ug/10^6 cells. Secondary antibody Goat anti-mouse IgG-PE at 0.25ug/10^6 cells. Both stable HEK293/hTLR4 and HEK293/Vector cell lines were equally stained.
|Tested species reactivity||Dog, Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Ba/F3 cell line expressing human TLR4 cell surface antigen|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||2-3 µg/10^6 cells|
|Functional Assay (FN)||Assay-Dependent|
|In vitro Assay (IV)||Assay-Dependent|
|Western Blot (WB)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
Suggested positive control: PBMCs or ThP1. This antibody is not recommended for Western Blot applications.
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The TLR4 cDNA codes for a protein consisting of 799 amino acids with approximate molecular weight of 88 kDa (6).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Human CD14 is an efficient target for recombinant immunoglobulin vaccine constructs that deliver T cell epitopes.
MA5-16205 was used in flow cytometry to assess targeting CD14 using recombinant Ig-based vaccines in order to elicit human T cell responses
|Tunheim G,Schjetne KW,Fredriksen AB,Sandlie I,Bogen B||Journal of leukocyte biology (77:303)||2005|