|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Extracellular region of murine TNF-R I.|
|Storage buffer||PBS with 0.1% BSA|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50|
|Functional Assay (FN)||1:50|
|Western Blot (WB)||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-40282 detects TNF-R I from mouse samples.
PA1-40282 has been successfully used in ELISA, functional assays, flow cytometry, immunoprecipitation, and Western blot applications.
The PA1-40282 imunogen is the extracellular region of mouse TNF-R I.
TNF-R I is a member of the TNF-receptor superfamily. This protein is one of the major receptors for the tumor necrosis factor-alpha. This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of this receptor were found to be associated with the autosomal dominant periodic fever syndrome. The impaired receptor clearance is thought to be a mechanism of the disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Loss of STAT3 in mouse embryonic fibroblasts reveals its Janus-like actions on mitochondrial function and cell viability.
PA1-40282 was used in western blot to study the role of STAT3 in mitochondrial function and cell viability using STAT3 knockout murine embryonic fibroblasts
|Zouein FA,Duhé RJ,Arany I,Shirey K,Hosler JP,Liu H,Saad I,Kurdi M,Booz GW||Cytokine (66:7)||2014|