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|Tested species reactivity||Human|
|Published species reactivity||Dog, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant human TNF-alpha.|
|Storage buffer||PBS, pH 7.5, with 5mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 6 publications below|
This product is recommended as a detection antibody in Sandwich ELISA applications.
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Production of antibodies to canine IL-1beta and canine TNF to assess the role of proinflammatory cytokines.||Baggio V,Ott F,Fischer RW,Gram H,Peele J,Spreng D,Schmökel H,Jungi TW||Veterinary immunology and immunopathology (107:27)||2005|
|Human||Not Cited||Synthetic TLR agonists reveal functional differences between human TLR7 and TLR8.||Gorden KB,Gorski KS,Gibson SJ,Kedl RM,Kieper WC,Qiu X,Tomai MA,Alkan SS,Vasilakos JP||Journal of immunology (Baltimore, Md. : 1950) (174:1259)||2005|
Differential regulation of CD44 expression by lipopolysaccharide (LPS) and TNF-alpha in human monocytic cells: distinct involvement of c-Jun N-terminal kinase in LPS-induced CD44 expression.
AHC3419 was used in ELISA to study CD44 in monocytic cells.
|Gee K,Lim W,Ma W,Nandan D,Diaz-Mitoma F,Kozlowski M,Kumar A||Journal of immunology (Baltimore, Md. : 1950) (169:5660)||2002|
Activation of the formyl peptide receptor by the HIV-derived peptide T-20 suppresses interleukin-12 p70 production by human monocytes.
AHC3419 was used in ELISA to study the mechanism of HIV-mediated suppression of IL-2 production.
|Braun MC,Wang JM,Lahey E,Rabin RL,Kelsall BL||Blood (97:3531)||2001|
Selective suppression of IL-12 production by chemoattractants.
AHC3419 was used in ELISA to determine the ability of chemoattractants to affect IL-2 production by human monocytes and dendritic cells.
|Braun MC,Lahey E,Kelsall BL||Journal of immunology (Baltimore, Md. : 1950) (164:3009)||2000|
Cholera toxin suppresses interleukin (IL)-12 production and IL-12 receptor beta1 and beta2 chain expression.
AHC3419 was used in ELISA to show that cholera toxin inhibits the production of interleukin-2.
|Braun MC,He J,Wu CY,Kelsall BL||The Journal of experimental medicine (189:541)||1999|
APC1 protein; Cachectin; macrophage-derived; member 2; monocyte-derived; TNF; TNF superfamily; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; TNF-alpha; TNFA; Tumor necrosis factor; tumor necrosis factor alpha; tumor necrosis factor ligand 1F; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
DIF; TNF; TNF-alpha; TNFA; TNFSF2; TNLG1F