|Tested species reactivity||Human|
|Published species reactivity||Dog, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant human TNF-alpha.|
|Storage buffer||PBS, pH 7.5, with 5mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 11 publications below|
This product is recommended as a detection antibody in Sandwich ELISA applications.
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Oligodeoxynucleotides differentially modulate activation of TLR7 and TLR8 by imidazoquinolines.
AHC3419 was used in ELISA to investigate the cross-talk between oligodeoxynucleotides, immune response modifiers, TLR7, and TLR8
|Gorden KK,Qiu X,Battiste JJ,Wightman PP,Vasilakos JP,Alkan SS||Journal of immunology (Baltimore, Md. : 1950) (177:8164)||2006|
|Not Applicable||Not Cited||
Apoptotic responses in squamous carcinoma and epithelial cells to small-molecule toll-like receptor agonists evaluated with automated cytometry.
AHC3419 was used in ELISA to develop an 96-well assay to quantitate DNA fragmentation in cells
|Inglefield JR,Larson CJ,Gibson SJ,Lebrec H,Miller RL||Journal of biomolecular screening (11:575)||2006|
|Production of antibodies to canine IL-1beta and canine TNF to assess the role of proinflammatory cytokines.||Baggio V,Ott F,Fischer RW,Gram H,Peele J,Spreng D,Schmökel H,Jungi TW||Veterinary immunology and immunopathology (107:27)||2005|
|Human||Not Cited||Synthetic TLR agonists reveal functional differences between human TLR7 and TLR8.||Gorden KB,Gorski KS,Gibson SJ,Kedl RM,Kieper WC,Qiu X,Tomai MA,Alkan SS,Vasilakos JP||Journal of immunology (Baltimore, Md. : 1950) (174:1259)||2005|
|Not Applicable||Not Cited||
The structure and biological characteristics of the Spirochaeta aurantia outer membrane glycolipid LGLB.
AHC3419 was used in ELISA to isolate and characterize LGLB from Spirochaeta aurantia
|Vinogradov E,Paul CJ,Li J,Zhou Y,Lyle EA,Tapping RI,Kropinski AM,Perry MB||European journal of biochemistry (271:4685)||2004|
Tumor necrosis factor-alpha induces functionally active hyaluronan-adhesive CD44 by activating sialidase through p38 mitogen-activated protein kinase in lipopolysaccharide-stimulated human monocytic cells.
AHC3419 was used in ELISA to suggest that TNF-alpha-induced p38 MAPK activation regulates the induction of functionally active HA-binding form of CD44 by activating sialidase in LPS-stimulated human monocytic cells.
|Gee K,Kozlowski M,Kumar A||The Journal of biological chemistry (278:37275)||2003|
Differential regulation of CD44 expression by lipopolysaccharide (LPS) and TNF-alpha in human monocytic cells: distinct involvement of c-Jun N-terminal kinase in LPS-induced CD44 expression.
AHC3419 was used in ELISA to study CD44 in monocytic cells.
|Gee K,Lim W,Ma W,Nandan D,Diaz-Mitoma F,Kozlowski M,Kumar A||Journal of immunology (Baltimore, Md. : 1950) (169:5660)||2002|
Activation of the formyl peptide receptor by the HIV-derived peptide T-20 suppresses interleukin-12 p70 production by human monocytes.
AHC3419 was used in ELISA to study the mechanism of HIV-mediated suppression of IL-2 production.
|Braun MC,Wang JM,Lahey E,Rabin RL,Kelsall BL||Blood (97:3531)||2001|
Effects of co-stimulation by CD58 on human T cell cytokine production: a selective cytokine pattern with induction of high IL-10 production.
AHC3419 was used in ELISA to examine the effects of co-stimulation by CD58 on the production of Th1-type or Th2 type cytokines.
|Bullens DM,Rafiq K,Charitidou L,Peng X,Kasran A,Warmerdam PA,Van Gool SW,Ceuppens JL||International immunology (13:181)||2001|
Selective suppression of IL-12 production by chemoattractants.
AHC3419 was used in ELISA to determine the ability of chemoattractants to affect IL-2 production by human monocytes and dendritic cells.
|Braun MC,Lahey E,Kelsall BL||Journal of immunology (Baltimore, Md. : 1950) (164:3009)||2000|
Cholera toxin suppresses interleukin (IL)-12 production and IL-12 receptor beta1 and beta2 chain expression.
AHC3419 was used in ELISA to show that cholera toxin inhibits the production of interleukin-2.
|Braun MC,He J,Wu CY,Kelsall BL||The Journal of experimental medicine (189:541)||1999|