|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 350-400 of human TRIAD3A.|
|Storage buffer||PBS with 0.2% gelatin|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: human spleen protein, antigen standard for RNF216 (transient overexpression lysate), human lung protein, human ovary protein, human pancreas protein, human skeletal muscle protein, human heart protein, human kidney protein, human liver protein, human brain protein, human brain tissue, heart tissue, kidney tissue, liver tissue, lung tissue, ovary tissue, pancreas tissue, small intestine tissue, skeletal muscle tissue, spleen tissue, human small intestine protein.
Principal Names: TRIAD3; E3-Ubiquitin Ligase TRIAD3; U7I1; ZIN; Zinc finger protein inhibiting NF-kappaB; Ubiquitin conjugating enzyme 7 interacting protein 1; UBCE7IP1; Official Gene Symbol: TRIAD3 Gene ID: 54476 (Human) Gene Map Locus: 7p22.1 (Human) TRIADs are novel zinc finger proteins characterized by the presence of Cysteine rich TRIAD domain consisting of DRIL motif flanked by 2 RING fingers. TRIAD3, a 56KDa protein, is a novel member of this family. It contains 4 RLD domains in the centre and a proline-rich domain at the C-terminus. Localized in the cytoplasm, it interacts with RIP and inhibits TNF and IL1-induced NF-kappaB activation pathways. Reports suggest TRIAD3 acts as an E3-ubiquitin-protein ligase and promotes ubiquitination and degradation of TLR4 and TLR9, thereby regulating TLR signaling. It also interacts with HIV type I Vif protein and interferes in the early events of HIV-1 replication.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.