Invitrogen

TRPV2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Reference

View all (14) TRPV2 antibodies

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Datasheet

Protocols

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TRPV2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis was performed in sections of mouse olfactory bulbs using TRPV2 Polyclonal Antibody (Product #OSR00190W). The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Primary antibody: dilution 1:2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

TRPV2 Antibody in Western Blot (WB) — Western blot analysis was performed on tissue lysates using TRPV2 Polyclonal Antibody (Product #OSR00190W). Blocking: 1% LFDM for 30 min at RT; primary antibody dilutions: in Muscle 1:4000; in Brain, Spleen, Skin: 1:3000; incubated at 4C overnight.

TRPV2 Antibody — TRPV2 siRNA triggered apoptotic cell death in leukemic blast cell lines. (A) Transmission (Top panels) and fluorescence (bottom panels) imaging showing successful transfection of K562 cells with fluorescein-coupled small interfering RNA (siRNA) targeting TRPV2. Negative siRNA represents a non-targeting siRNA control. Efficiency of TRPV2 gene silencing was determined 24?h after transfection by (B) qRT-PCR (performed with the set of primers to detect all TRPV2 isoforms), and (C) Western blot. TRPV2 gene silencing-induced apoptosis in (D) K562 (*P?

Product Details

OSR00190W

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:2,000	-

Product Specifications
Species Reactivity	Human, Mouse, Non-human primate, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthetic peptide from human TRPV2 conjugated to blue carrier protein was used as the antigen.
Conjugate	Unconjugated
Form	Lyophilized
Concentration	Conc. Not Determined
Storage buffer	whole serum
Contains	no preservative
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
RRID	AB_962458

Product Specific Information

Glycerol (1:1) may be added for additional stability.

Reconstitute with 100 uL of sterile water

Target Information

VLR-1 is a calcium-permeable, non-selective cation channel with an outward rectification, that seems to be regulated, at least in part, by growth factors, like IGF1, PDGF and morphogenetic neuropeptide/head activator. VRL-1 does not respond to capsaicin, acid, or moderate heat, but instead is activated by high temperatures, with a threshold of approximately 52 degrees C. Within sensory ganglia, VRL-1 is most prominently expressed by a subset of medium- to large-diameter neurons, making it a candidate receptor for transducing high-threshold heat responses in this class of cells. VRL-1 transcripts are not restricted to the sensory nervous system, indicating that this channel may be activated by stimuli other than heat. VRL-1 is one of the six members of the vanilloid-like TRP-channel family which is now known as TRPV family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Evaluation of cationic channel TRPV2 as a novel biomarker and therapeutic target in Leukemia-Implications concerning the resolution of pulmonary inflammation.

Scientific reports

Siveen KS,Prabhu KS,Parray AS,Merhi M,Arredouani A,Chikri M,Uddin S,Dermime S,Mohammad RM,Steinhoff M,Janahi IA,Azizi F

OSR00190W was used in Western Blotting to demonstrate that transient receptor potential vanilloid type 2 channel (TRPV2), reputed for its role in inflammatory processes, exhibited oncogenic activity associated with alteration of its molecular expression profile.

Thu Feb 07 00:00:00 EST 2019

Bioinformatics

Protein Aliases: GRC; growth factor regulated channel; Growth factor-regulated calcium channel; Osm-9-like TRP channel 2; OTRPC2; RP23-234K24.7; Stretch-activated channel 2B; Transient receptor potential cation channel subfamily V member 2; transient receptor potential cation channel, subfamily V, member 2; TrpV2; Vanilloid receptor-like protein 1; VRL-1

Gene Aliases: GRC; OTRPC2; Sac2b; TRPV2; VRL; VRL-1; VRL1

UniProt ID: (Human) Q9Y5S1, (Mouse) Q9WTR1, (Rat) Q9WUD2

Entrez Gene ID: (Human) 51393, (Mouse) 22368, (Rat) 29465

Molecular Function: ion channel