Invitrogen

TRPV2 Polyclonal Antibody

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TRPV2 Antibody in Western Blot (WB) — Western blot analysis was performed on tissue lysates using TRPV2 Polyclonal Antibody (Product #OSR00190W). Blocking: 1% LFDM for 30 min at RT; primary antibody dilutions: in Muscle 1:4000; in Brain, Spleen, Skin: 1:3000; incubated at 4C overnight.

TRPV2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis was performed in sections of mouse olfactory bulbs using TRPV2 Polyclonal Antibody (Product #OSR00190W). The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Primary antibody: dilution 1:2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:300 - 1:2,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:2,000

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide from human TRPV2 conjugated to blue carrier protein was used as the antigen.

Conjugate

Unconjugated

Form

Lyophilized

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

RRID

AB_962458

Product Specific Information

Glycerol (1:1) may be added for additional stability.

Reconstitute with 100 uL of sterile water

Target Information

VLR-1 is a calcium-permeable, non-selective cation channel with an outward rectification, that seems to be regulated, at least in part, by growth factors, like IGF1, PDGF and morphogenetic neuropeptide/head activator. VRL-1 does not respond to capsaicin, acid, or moderate heat, but instead is activated by high temperatures, with a threshold of approximately 52 degrees C. Within sensory ganglia, VRL-1 is most prominently expressed by a subset of medium- to large-diameter neurons, making it a candidate receptor for transducing high-threshold heat responses in this class of cells. VRL-1 transcripts are not restricted to the sensory nervous system, indicating that this channel may be activated by stimuli other than heat. VRL-1 is one of the six members of the vanilloid-like TRP-channel family which is now known as TRPV family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: GRC; growth factor regulated channel; Growth factor-regulated calcium channel; Osm-9-like TRP channel 2; OTRPC2; RP23-234K24.7; Stretch-activated channel 2B; Transient receptor potential cation channel subfamily V member 2; transient receptor potential cation channel, subfamily V, member 2; TrpV2; Vanilloid receptor-like protein 1; VRL-1

Gene Aliases: GRC; OTRPC2; Sac2b; TRPV2; VRL; VRL-1; VRL1

UniProt ID: (Human) Q9Y5S1, (Mouse) Q9WTR1, (Rat) Q9WUD2

Entrez Gene ID: (Human) 51393, (Mouse) 22368, (Rat) 29465

Molecular Function: ion channel transporter