Invitrogen

Tau (Cleaved Asp421, Asp422) Monoclonal Antibody (TauC3)

12 Published Figures

11 References

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Protocols

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Datasheet

Protocols

Questions & Answers

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Tau (Cleaved Asp421, Asp422) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Tau (Cleavage site 421/422) Antibody (TauC3) was done on 70% confluent log phase SHSY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Tau (Cleavage site 421/422) Antibody (TauC3) (Product # AHB0061) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (Product # A12381). Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 40X magnification.

Tau (Cleaved Asp421, Asp422) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Tau (421/422 cleavage site) showing staining in the cytoplasm of paraffin-embedded mouse brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Tau (421/422 cleavage site) monoclonal antibody (Product # AHB0061) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Tau (Cleaved Asp421, Asp422) Antibody in Immunohistochemistry (IHC) — Figure 7 Tau pathology in pericyte-deficient APP sw/0 Pdgfrbeta +/- mice. ( a - f ) Representative cortex and hippocampus sections stained with antibodies against p-tau (Thr231) ( a ), quantification of p-tau (Thr231)-positive neurons ( b ), p-tau (Ser202/Thr205, AT8) ( c ), quantification of AT8-positive neurons ( d ), caspase-cleaved tau ( e ) and an early pathological tau marker MC1 ( f ) in 9-month-old APP sw/0 Pdgfrbeta +/- mice and age-matched APP sw/0 Pdgfrbeta +/+ littermates. In a , c , e , f , scale bar, 25 mum. CA1 and CA3 denote hippocampal regions. In b , d , values are means+-s.e.m., n =5 mice per group. P <0.05 by ANOVA followed by Tukey's post-hoc tests.

Tau (Cleaved Asp421, Asp422) Antibody in Western Blot (WB) — Figure 4 miR-485-3p antisense oligonucleotide (ASO) reduces apoptosis and truncated tau level. ( A ) Immunoblotting for NeuN and cleaved caspase-3 expression in fractions from the cortex region of control- or miR-485-3p ASO-injected 10-month-old 5XFAD mice. Data are representative of three independent experiments. ( B , C ) Relative protein quantification of NeuN ( B ) and cleaved caspase-3 ( C ) obtained from ( A ). Data obtained across four independent experiments are expressed as mean +- SD. ( D ) Immunohistochemical staining for NeuN and cleaved caspase-3 in coronal brain sections from control- or miR-485-3p ASO-injected 10-month-old 5XFAD mice. Scale bars, 20 mum. Data are representative of three independent experiments. ( E ) Quantification of the NeuN + cleaved caspase-3 + cells from control- ( n = 4) or miR-485-3p ASO- ( n = 5) injected 10-month-old 5XFAD mice. ( F ) Primary cortical neurons were treated with 1 muM oligomeric Abeta (1-42) for 6 h; lysates were immunoblotted with cleaved tau or cleaved caspase-3 antibodies. Data are representative of three independent experiments. ( G ) Immunoblotting for cleaved tau protein expression in control- ( n = 3) or miR-485-3p ASO- ( n = 5) injected 10-month-old 5XFAD mice. ( H ) Relative protein quantification of cleaved tau obtained from ( G ). Data obtained over three independent experiments are expressed as mean +- SD. ** p < 0.01; *** p < 0.001 (two-tailed t -test).

Product Details

AHB0061

Applications	Tested Dilution	Publications
Western Blot (WB)	Assay-dependent	View 8 publications 8 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:10-1:100	-
Immunocytochemistry (ICC/IF)	1 µg/mL	-
Immunoprecipitation (IP)	Assay-dependent	-
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	TauC3
Immunogen	KLH-CSSTGSIDMVD, corresponding to the C terminus of tau truncated at Asp421. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 1% BSA
Contains	0.1% sodium azide
Storage conditions	-20°C
RRID	AB_2536237

Product Specific Information

This antibody recognizes tau when truncated at Asp421. Several caspases, including caspase-3, caspase-7, and caspase-8, cleave tau at this site, yielding the truncated form of the protein. This antibody does not react with full-length tau, or with tau truncated at Glu391 or Ala429. This antibody provides a tool for studying neuronal cell apoptosis.

Recommended Positive Control: Tau and caspase-3 cleaved tau.

Target Information

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclindependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Relevance of Phosphorylation and Truncation of Tau to the Etiopathogenesis of Alzheimer's Disease.

Frontiers in aging neuroscience

Zhou Y,Shi J,Chu D,Hu W,Guan Z,Gong CX,Iqbal K,Liu F

Published figure using Tau (Cleaved Asp421, Asp422) monoclonal antibody (Product # AHB0061) in Western Blot

Sat Apr 09 00:00:00 EDT 2022

Targeting MicroRNA-485-3p Blocks Alzheimer's Disease Progression.

International journal of molecular sciences

Koh HS,Lee S,Lee HJ,Min JW,Iwatsubo T,Teunissen CE,Cho HJ,Ryu JH

Published figure using Tau (Cleaved Asp421, Asp422) monoclonal antibody (Product # AHB0061) in Western Blot

Sat Dec 04 00:00:00 EST 2021

Early-life exposure to high-fat diet influences brain health in aging mice.

Aging cell

Di Meco A,Praticò D

Published figure using Tau (Cleaved Asp421, Asp422) monoclonal antibody (Product # AHB0061) in Western Blot

Sun Dec 01 00:00:00 EST 2019

Early-life exposure to high-fat diet influences brain health in aging mice.

Aging cell

Di Meco A,Praticò D

Published figure using Tau (Cleaved Asp421, Asp422) monoclonal antibody (Product # AHB0061) in Western Blot

Sun Dec 01 00:00:00 EST 2019

Early-life exposure to high-fat diet influences brain health in aging mice.

Aging cell

Di Meco A,Praticò D

Published figure using Tau (Cleaved Asp421, Asp422) monoclonal antibody (Product # AHB0061) in Western Blot

Sun Dec 01 00:00:00 EST 2019

A unique tau conformation generated by an acetylation-mimic substitution modulates P301S-dependent tau pathology and hyperphosphorylation.

The Journal of biological chemistry

Ajit D,Trzeciakiewicz H,Tseng JH,Wander CM,Chen Y,Ajit A,King DP,Cohen TJ

AHB0061 was used in Western Blotting to indicate that a complex tau PTM code likely regulates tau pathogenesis, highlighting the potential utility of manipulating and detoxifying tau strains through site-specific tau-targeting approaches.

Fri Nov 08 00:00:00 EST 2019

Pimozide reduces toxic forms of tau in TauC3 mice via 5' adenosine monophosphate-activated protein kinase-mediated autophagy.

Journal of neurochemistry

Kim YD,Jeong EI,Nah J,Yoo SM,Lee WJ,Kim Y,Moon S,Hong SH,Jung YK

AHB0061 was used in Western Blotting to study the mechanism by which pimozide can rescue tau pathology in an Alzheimer's disease model.

Fri Sep 01 00:00:00 EDT 2017

Subacute Changes in Cleavage Processing of Amyloid Precursor Protein and Tau following Penetrating Traumatic Brain Injury.

PloS one

Cartagena CM,Mountney A,Hwang H,Swiercz A,Rammelkamp Z,Boutte AM,Shear DA,Tortella FC,Schmid KE

Published figure using Tau (Cleaved Asp421, Asp422) monoclonal antibody (Product # AHB0061) in Western Blot

Wed Jul 26 00:00:00 EDT 2017

Subacute Changes in Cleavage Processing of Amyloid Precursor Protein and Tau following Penetrating Traumatic Brain Injury.

PloS one

Cartagena CM,Mountney A,Hwang H,Swiercz A,Rammelkamp Z,Boutte AM,Shear DA,Tortella FC,Schmid KE

Published figure using Tau (Cleaved Asp421, Asp422) monoclonal antibody (Product # AHB0061) in Western Blot

Wed Jul 26 00:00:00 EDT 2017

Modifications of tau protein after cerebral ischemia and reperfusion in rats are similar to those occurring in Alzheimer's disease - Hyperphosphorylation and cleavage of 4- and 3-repeat tau.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Fujii H,Takahashi T,Mukai T,Tanaka S,Hosomi N,Maruyama H,Sakai N,Matsumoto M

AHB0061 was used in Western Blotting to study the pathological association of tau dynamics in both cerebral ischemia and Alzheimer's disease.

Sat Jul 01 00:00:00 EDT 2017

The identification of raft-derived tau-associated vesicles that are incorporated into immature tangles and paired helical filaments.

Neuropathology and applied neurobiology

Nishikawa T,Takahashi T,Nakamori M,Hosomi N,Maruyama H,Miyazaki Y,Izumi Y,Matsumoto M

AHB0061 was used in immunohistochemistry - paraffin section to study the relationship between neurofibrillary tangles and raft domains

Thu Dec 01 00:00:00 EST 2016

Carboxy terminus heat shock protein 70 interacting protein reduces tau-associated degenerative changes.

Journal of Alzheimer's disease : JAD

Saidi LJ,Polydoro M,Kay KR,Sanchez L,Mandelkow EM,Hyman BT,Spires-Jones TL

AHB0061 was used in western blot to test if CHIP ameliorates the pathological changes associated with tau aggregation.

Mon Oct 26 00:00:00 EDT 2015

Pericyte loss influences Alzheimer-like neurodegeneration in mice.

Nature communications

Sagare AP,Bell RD,Zhao Z,Ma Q,Winkler EA,Ramanathan A,Zlokovic BV

Published figure using Tau (Cleaved Asp421, Asp422) monoclonal antibody (Product # AHB0061) in Immunohistochemistry

Tue Jul 08 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: G protein beta1/gamma2 subunit-interacting factor 1; map tau; Microtubule-associated protein tau; microtubule-associated protein tau, isoform 4; microtubules; Neurofibrillary tangle protein; neurofibrillary tangles; Paired helical filament-tau; PHF-tau; protein phosphatase 1, regulatory subunit 103; Tau microtubule-associated protein

Gene Aliases: AI413597; AW045860; DDPAC; FTDP-17; MAPT; MAPTL; MSTD; Mtapt; MTBT1; MTBT2; PPND; PPP1R103; pTau; RNPTAU; TAU

UniProt ID: (Human) P10636, (Mouse) P10637

Entrez Gene ID: (Human) 4137, (Rat) 29477, (Mouse) 17762

Function(s)

microtubule cytoskeleton organization neuron migration brain development female pregnancy response to nutrient adult walking behavior axo-dendritic transport intrinsic apoptotic signaling pathway in response to oxidative stress response to organic substance regulation of autophagy regulation of microtubule polymerization positive regulation of microtubule polymerization negative regulation of intracellular transport positive regulation of axon extension mitochondrion transport along microtubule axon extension generation of neurons regulation of microtubule-based movement apoptotic process microtubule-based process axonogenesis neuron differentiation

Process(es)

structural constituent of cytoskeleton protein binding microtubule binding SH3 domain binding enzyme binding protein kinase binding protein complex binding apolipoprotein binding protein phosphatase 2A binding lipoprotein particle binding protein domain specific binding tubulin binding

Tau (Cleaved Asp421, Asp422) Monoclonal Antibody (TauC3)

Product Details

AHB0061

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Immunohistochemistry (IHC)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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