|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgM|
|Immunogen||Asialo ovine submaxillary mucin (AOSM).|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:50-1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-90544 detects Tn antigen from human samples. MA1-90544 stains Tn antigen in epithelial carcinoma, pancreatic, colon, stomach and breast.
MA1-90544 has been successfully used in immunocytochemistry, immunohistochemistry (frozen tissue), and immunohistochemistry (paraffin tissue) applications. A suggested positive control for this produt is human gastrointestinal tumor.
The MA1-90544 immunogen is Asialo ovine submaxillary mucin (AOSM).
Aberrant glycosylation is a hallmark of cancer cells. The blood group precursors T (Thomsen-Friedenreich) and Tn epitopes are shielded in healthy and benign-diseased tissues but uncovered in approximately 90% of carcinomas. T and Tn glycoproteins are specific, autoimmunogenic pancarcinoma antigens. These antigens may also be found in neoplastic blood cells. Fundamental, chemical and physical aspects of these glycoproteins of primary carcinomas are discussed first. Tn and T epitopes are cell and tissue adhesion molecules, essential in invasion by and metastasis of carcinoma which includes adherent and proliferative phases. Immunohistochemical studies of the extent of Tn antigen expression in primamry breast carcinoma demonstrate its highly significant correlation with clinical pathological tumor stages, and hence its value as a reliable prognisticator.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.