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Western blot analysis of cell lysates using mouse anti-topoisomerase I antibody (Catalog no. 43-5900). HeLa cell lysates (lanes 1-3) after immunoprecipitation (lane 1), negative immunoprecipitation control (lane 2); no immunoprecipitation (lane 3); BT474 cell lysates (lane 4); T47D cell lysates.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant protein derived from the N-terminal region of human TOP1 protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Topoisomerases are nuclear enzymes involved in a variety of cellular activities such as chromosome condensation, DNA replication, transcription, recombination and segregation at mitosis. Human topoisomerase I is a 100kDa protein capable of relaxing positively and negatively supercoiled DNA by performing a transient single stranded nick which is then relegated at the end of the reaction. It has been shown that the enzyme is located in regions of the genome that are undergoing active RNA synthesis, where it probably reduces superhelical stresses in the DNA, enabling RNA polymerase to function properly. Both DNA topoisomerases I and II have been found to be targets of autoantibodies in the sera of patients with certain autoimmune diseases such as systemic lupus erythematosus, and also of some anti tumor drugs and antibiotics. Elevated levels of DNA topoisomerase I, detected by transfer assays, have been demonstrated in colorectal tumors compared with normal colon mucosa as a result of increased transcription or mRNA stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
TOPI; type I DNA topoisomerase