|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG|
|Immunogen||Purified trKB receptors from insect cells (sf9) transfected with Baculovirus.|
|Storage buffer||0.1M tris HCl, pH 7.5|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-91843 detects TrkB from human samples.
MA1-91843 has been successfully used in immunoprecipitation and Western blot applications. The predicted molecular weight is 92 kDa.
The MA1-91843 immunogen is: Purified trKB receptors from insect cells (sf9) transfected with Baculovirus.
Neurotrophins activate tyrosine kinase receptors of the trk family (trkA, trkB and trkC) and they all share a common low affinity receptor (p75NTR or p75). Although p75NTR does not appear to directly transduce NGF signals, this receptor appears to increase the responsiveness of the trk receptors and plays a role in the retrograde transport of neurotrophin signals from axon terminals to the cell body. The expression of trk gene neurotrophin receptors in mouse is tabulated by Barbacid (1994). NGF binds with high affinity and activates the TrkA tyrosine kinase receptor, which is apparently responsible for signal transduction. BDNF activates the TrkB tyrosine kinase receptor, which it shares with NT-4. NT-3 activates the TrkC tyrosine kinase receptor, but can also activate TrkA and TrkB receptors in certain cell systems. NT-4 primarily activates the TrkB tyrosine kinase receptor, which it shares with BDNF.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.