|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Native human cardiac troponin I, purity greater than 98%.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Cardiac-specific troponin I (TnI) is an integral inhibitory protein of cardiac muscle which exists as a complex with troponin C (TnC) and troponin T (TnT). The TnT subunit of troponin binds to tropomyosin t form a troponin-tropomyosin complex, anchored in place by the binding of TnI to actin, within muscle thin filaments. Structural change resulting from the binding of calcium to specific sites on the regulatory TnC subunit, releases the inhibitory region of TnI from actin, anabling the attachment of the molecular motor protein myosin, allowing for muscle contraction and hence movement. The measurements of blood TnI and TnT levels is an important diagnostics indicator of heart muscle damage, and can be used to differentiate between angina and myocardic infarction in patients with chest pains. Troponin I has also been shown to inhibit angiogenesis in vivo and in vitro.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.