Immunohistochemistry analysis of Tuberin / TSC2 showing staining in the cytoplasm of paraffin-embedded human liver tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Tuberin / TSC2 Mouse Monoclonal Antibody (37-0500) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human tuberin protein|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Mutations in TSC2 lead to tuberous sclerosis complex. The protein is believed to be a tumor suppressor and is able to specifically stimulate the intrinsic GTPase activity of the Ras-related protein RAP1A and RAB5. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. TSC2 may have a function in vesicular transport, but may also play a role in the regulation of cell growth arrest and in the regulation of transcription mediated by steroid receptors. Interaction between TSC1 and TSC2 may facilitate vesicular docking.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Death-associated protein kinase 1 promotes growth of p53-mutant cancers.
37-0500 was used in immunoprecipitation to identify molecular targets that suppress tumorigenesis in triple negative breast cancers.
|Zhao J,Zhao D,Poage GM,Mazumdar A,Zhang Y,Hill JL,Hartman ZC,Savage MI,Mills GB,Brown PH||The Journal of clinical investigation (125:2707)||2015|
|Human||Not Cited||TBC1D7 is a third subunit of the TSC1-TSC2 complex upstream of mTORC1.||Dibble CC,Elis W,Menon S,Qin W,Klekota J,Asara JM,Finan PM,Kwiatkowski DJ,Murphy LO,Manning BD||Molecular cell (47:535)||2012|
Clinicopathological and immunohistochemical findings in an autopsy case of tuberous sclerosis complex.
37-0500 was used in immunohistochemistry - paraffin section to report the neuropathological findings of a 32-year-old patient with a germ-line mutation in the TSC2 gene
|Boer K,Troost D,Jansen F,Nellist M,van den Ouweland AM,Geurts JJ,Spliet WG,Crino P,Aronica E||Neuropathology : official journal of the Japanese Society of Neuropathology (28:577)||2008|