Immunofluorescence analysis of Tubulin Beta was performed using 70% confluent log phase NIH/3T3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Tubulin Beta Rabbit Polyclonal Antibody (PA516863) at 2 ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Amphibian, Bovine, Chicken, Fungi, Gerbil, Guinea pig, Human, Mouse, Pig, Rat, Sea urchin, Yeast|
|Published species reactivity||Yeast, Rat, Sheep, Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide derived from aa 416-430 of human tubulin beta|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2-3 µg/ml|
|Immunofluorescence (IF)||2-3 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16863 targets Tubulin Beta in IHC (P), WB, and IP applications and shows reactivity with Amphibian, Bovine, Chicken, Fungi, Gerbil, Guinea Pig, Human, mouse, Porcine, Rat, Sea Urchin, and Yeast samples.
The PA5-16863 immunogen is a synthetic peptide derived from aa 416-430 of human tubulin beta.
Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55kDa subunits: alpha and beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Genetic Evidence for the Role of the Vacuole in Supplying Secretory Organelles with Ca2+ in Hansenula polymorpha.
PA5-16863 was used in western blot to study calcium deficiency in the secretory pathway caused by Pmr1 inactivation
|Fokina AV,Chechenova MB,Karginov AV,Ter-Avanesyan MD,Agaphonov MO||PloS one (10:null)||2015|
Fetal inhibition of inflammation improves disease phenotypes in harlequin ichthyosis.
PA5-16863 was used in western blot to examine the developing epidermis to determine the consequences of lipid dysregulation in mouse models.
|Cottle DL,Ursino GM,Ip SC,Jones LK,Ditommaso T,Hacking DF,Mangan NE,Mellett NA,Henley KJ,Sviridov D,Nold-Petry CA,Nold MF,Meikle PJ,Kile BT,Smyth IM||Human molecular genetics (24:436)||2015|
N-Cadherin is a prospective cell surface marker of human mesenchymal stem cells that have high ability for cardiomyocyte differentiation.
PA5-16863 was used in western blot to study the value of N-cadherin expression as a prospective marker of human mesenchymal stem cells that have a high probability of differentiating into cardiomyocytes
|Ishimine H,Yamakawa N,Sasao M,Tadokoro M,Kami D,Komazaki S,Tokuhara M,Takada H,Ito Y,Kuno S,Yoshimura K,Umezawa A,Ohgushi H,Asashima M,Kurisaki A||Biochemical and biophysical research communications (438:753)||2013|
Heterogeneous time-dependent response of adipose tissue during the development of cancer cachexia.
PA5-16863 was used in western blot to study cancer cachexia and the temporal response of different adipose tissue depots
|Batista ML,Neves RX,Peres SB,Yamashita AS,Shida CS,Farmer SR,Seelaender M||The Journal of endocrinology (215:363)||2012|
Expression of proto-oncogene KIT is up-regulated in subset of human meningiomas.
PA5-16863 was used in western blot to study the upregulation of the proto-oncogene KIT in a subset of human meningiomas
|Saini M,Jha AN,Abrari A,Ali S||BMC cancer (12:null)||2012|
A subset of human gliomas shows over-expression of KIT without its amplification.
PA5-16863 was used in western blot to study the overexpression of KIT in the absence of gene amplification as observed in a subset of human glioma patients
|Saini M,Jha AN,Abrari A,Ali S||Gene (497:155)||2012|
Chronic pulsatile hyperglycemia reduces insulin secretion and increases accumulation of reactive oxygen species in fetal sheep islets.
PA5-16863 was used in western blot to study insulin secretion and ROS accumulation in pancreatic islets from fetal sheep subjected to chronic pulsed hyperglycemia
|Green AS,Chen X,Macko AR,Anderson MJ,Kelly AC,Hart NJ,Lynch RM,Limesand SW||The Journal of endocrinology (212:327)||2012|
Phospholipase C-related catalytically inactive protein (PRIP) controls KIF5B-mediated insulin secretion.
PA5-16863 was used in immunocytochemistry to study the role of phospholipase C-related catalytically inactive protein in KIF5B-mediated insulin secretion
|Asano S,Nemoto T,Kitayama T,Harada K,Zhang J,Harada K,Tanida I,Hirata M,Kanematsu T||Biology open (3:463)||2014|
Neurite outgrowth of mature retinal ganglion cells and PC12 cells requires activity of CK1¿ and CK1¿.
PA5-16863 was used in immunocytochemistry to determine the role of CK1delta and CK1epsilon activity in neurite outgrowth of lens injury stimulated retinal ganglion cells and nerve growth factor stimulated PC12 cells
|Bischof J,Müller A,Fänder M,Knippschild U,Fischer D||PloS one (6:null)||2011|