Immunofluorescent analysis of Tyrosine Hydroxylase using a Tyrosine Hydroxylase monoclonal antibody (Product # MA1-24654). Immunoperoxidase (A and C) and immunofluorescent (B, D–H) labeling of intrinsic cardiac adrenergic (ICA) cells in human hearts are shown. ICA cells expressing tyrosine hydroxylase (TH) immunoreactivity (red) are distributed diffusely throughout the left ventricular (LV) myocardium. Perivascular location is a frequent feature of ICA cells. C, arrow: terminal arteriole. E: abundant ICA cells in the smooth muscle layers of epicardial circumflex coronary artery. TH-expressing sympathetic nerve fibers (D and G, arrows) can occasionally be seen in the field. B and D, insets: magnified ICA cell images (arrows). TH immunoreactivity (green) was identified in ICA cells and sympathetic nerve fibers in the sinoatrial nodal tissue (G). ICA cells are seen in transplanted human LV tissue (H). Scale bars = 10 µm, except in B (20 µm).
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant protein corresponding to the C-terminal portion of mouse Tyrosine Hydroxylase.|
|Storage buffer||tissue culture supernatant|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:20-1:40|
|Western Blot (WB)||1:25-1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 5 publications below|
MA1-24654 detects Tyrosine Hydroxylase from human, mouse, and rat samples.
MA1-24654 has been successfully used in Western blot, ICC/IF, and immunohistochemistry (frozen sections) procedures.
The MA1-24654 immunogen is recombinant protein corresponding to the C-terminal portion of mouse Tyrosine Hydroxylase.
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamine neurotransmitters (dopamine, epinephrine, and norepinephrine). It is responsible for the conversion of L-tyrosine to L-dopa in the catecholamine synthesis pathway. In all species, catecholamine synthesis is regulated by the interaction of TH with a cofactor, tetrahydrobiopterin (BH4). BH4 binds to the TH catalytic domain, resulting in enzymatic activity. Unlike TH in non-primate species, four human TH mRNA splice variants (hTH1-hTH4) have been isolated. These variants are identical in their catalytic domain, but differ in their N-terminal, regulatory domains. Little information has been uncovered regarding the regulatory role of these isoforms in vivo.
The role of TH in the synthesis of catecholamine neurotransmitters suggests a correlation between the enzyme and a number of neuropathogenic diseases characterized by irregular catecholamine levels. Catecholamine level irregularities have been uncovered in Parkinson's disease, schizophrenia, and dystonia, as well as a variety of cardiovascular diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Melatonin alterations and brain acetylcholine lesions in sleep disorders in Cockayne syndrome.
MA1-24654 was used in immunohistochemistry to study Cockayne syndrome sleep disorders and the roles played by altered melatonin metabolism and loss of acetylcholine neurons
|Okoshi Y,Tanuma N,Miyata R,Hayashi M||Brain and development (36:907)||2014|
An autopsy case of microencephaly, bizarre putaminal lesion, and cerebellar atrophy with heart and liver diseases.
MA1-24654 was used in immunohistochemistry to report on an autopsy case displaying microencephaly, putaminal lesion, and cerebellar atrophy plus heart and liver disease
|Okoshi Y,Hayashi M,Kanda S,Yamamoto T||Brain and development (36:707)||2014|
Lesions of acetylcholine neurons in refractory epilepsy.
MA1-24654 was used in immunohistochemistry to study the presence of lesions in acetylcholinergic neurons in various types of refractory epilepsy
|Hayashi M,Nakajima K,Miyata R,Tanuma N,Kodama T||ISRN neurology (2012:null)||2012|
Intestinal epithelial stem/progenitor cells are controlled by mucosal afferent nerves.
MA1-24654 was used in immunohistochemistry to investigate the role of mucosal afferent nerves in the maintenance and differentiation of intestinal epithelial progenitor cells
|Lundgren O,Jodal M,Jansson M,Ryberg AT,Svensson L||PloS one (6:null)||2011|
An autopsy case presenting repetitive hypoglycemia and unique cortical dysplasia.
MA1-24654 was used in immunohistochemistry to investigate a case with repetitive hypoglycemia and unique cortical dysplasia
|Hayashi M,Hachiya Y,Arai N||Brain and development (32:289)||2010|