|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptides corresponding to residues 2-19 and 131-148 of human UBE2N.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: antigen standard for UBE2N (transient overexpression lysate), human heart tissue, human heart protein.
UBE2N (ubiquitin-conjugating enzyme E2N) is a novel human ubiquitin-conjugating enzyme with an ability to form a thiol ester linkage with ubiquitin in an ubiquitin-activating enzyme E1-dependent manner. The amino acid sequence showed 80% identity with the Drosophila's bendless gene product (ubiquitin-conjugating enzyme E2). The corresponding transcripts are highly expressed in heart, skeletal muscle, and testis. UBE2N protein may be involved in protein degradation mainly in the muscles and testis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.