OriGene

USP10 Monoclonal Antibody (OTI1A10), TrueMAB™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

View all (27) USP10 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

USP10 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human breast tissue within the normal limits using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504130)

USP10 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP10 (RC200835, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-USP10. Positive lysates LY417493 (100 µg) and LC417493 (20 µg) can be purchased separately from OriGene.

USP10 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of Jurkat cells, using anti-USP10 antibody (TA504130), (Red), compared to a nonspecific negative control antibody, (Blue).

USP10 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in USP10 knockout (KO) Hela cell line using Anti-USP10 Monoclonal Antibody (OTI1A10), TrueMAB™ (Product # TA504130). {KO}

Product Details

TA504130

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:150	-
Flow Cytometry (Flow)	1:100	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	OTI1A10
Immunogen	Full length human recombinant protein of human USP10 produced in HEK293T cell. View antigen
Conjugate	Unconjugated
Form	liquid
Concentration	0.92 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Target Information

USP10, also known as ubiquitin specific peptidase 10, belongs to the ubiquitin-specific protease family of cysteine proteases. USP10 functions to catalyze the cleavage of ubiquitin from ubiquitin-conjugated protein substrates such as p53/TP53, SNX3 and CFTR. USP10 has been identified as a subunit of DNA-bound androgen receptor (AR) complexes and may play a role in the activity of the DNA-bound androgen receptor complex. USP10 also acts as an essential regulator of p53/TP53 stability and is thought to function as a tumor suppressor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Deubiquitinating enzyme 10; MGC2621; Ubiquitin carboxyl-terminal hydrolase 10; ubiquitin specific protease 10; Ubiquitin thioesterase 10; ubiquitin thiolesterase 10; Ubiquitin-specific-processing protease 10

Gene Aliases: KIAA0190; UBPO; USP10

UniProt ID: (Human) Q14694

Entrez Gene ID: (Human) 9100

Function(s)

ubiquitin-dependent protein catabolic process autophagy cellular response to DNA damage stimulus regulation of autophagy protein deubiquitination translesion synthesis DNA damage response, signal transduction by p53 class mediator negative regulation of I-kappaB kinase/NF-kappaB signaling cellular response to interleukin-1

Process(es)

p53 binding cysteine-type endopeptidase activity thiol-dependent ubiquitin-specific protease activity protein binding ion channel binding poly(A) RNA binding