Immunohistochemistry analysis of Ubiquilin showing staining in the nucleus and weak staining in the cytoplasm of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Ubiquilin Rabbit Polyclonal Antibody (PA1-759) diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse, Hamster|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: A(2) E S A E S G G P P G A Q D S A A(18)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:100|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
PA1-759 detects ubiquilin from hamster cells.
PA1-759 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~63 kDa protein representing ubiquilin from CHO cell extract.
PA1-759 immunizing peptide corresponds to amino acids residues 2-18 from mouse ubiquilin.
Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer and quote;s disease. This peptide is generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP by an aspartyl protease (BACE). Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. Gamma-secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. Associated with the presenilins are nicastrin and ubiquilin: nicastrin has been found to bind to the carboxyl-terminus of beta APP and helps to modulate the production of the amyloid beta peptide. Ubiquilin is believed to promote presenilin protein accumulation. Also found in the neurofibrillary lesions associated with Alzheimer and quote;s disease is Tau, a neuronal microtubule-associated protein found predominantly on axons that functions to promote tubulin polymerization and stabilize microtubules.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Signature changes in ubiquilin expression in the R6/2 mouse model of Huntington's disease.
PA1-759 was used in immunocytochemistry and western blot to evaluate the diagnostic significance of ubiquilin expression in Huntington disease
|Safren N,Chang L,Dziki KM,Monteiro MJ||Brain research (1597:37)||2015|
Effects of ubiquilin 1 on the unfolded protein response.
PA1-759 was used in western blot to investigate the influence of ubiquilin 1 on the unfolded protein response and its role in Alzheimer disease
|Lu A,Hiltunen M,Romano DM,Soininen H,Hyman BT,Bertram L,Tanzi RE||Journal of molecular neuroscience : MN (38:19)||2009|
Ubiquilin 1 modulates amyloid precursor protein trafficking and Abeta secretion.
PA1-759 was used in western blot to investigate the role of ubiquilin 1 during the amyloid precursor protein trafficking and Abeta secretion.
|Hiltunen M,Lu A,Thomas AV,Romano DM,Kim M,Jones PB,Xie Z,Kounnas MZ,Wagner SL,Berezovska O,Hyman BT,Tesco G,Bertram L,Tanzi RE||The Journal of biological chemistry (281:32240)||2006|