Western blot analysis of VEGF Receptor 2/Flk-1 in culture supernatant extract of baculovirus exp. H.VEGFR-2. Samples were probed with a VEGF Receptor 2/Flk-1 monoclonal antibody (Product # MA1-24888). The antibody was developed with Alk Phos APA mouse Fab Ads HIgG and a NBT/BCIP substrate.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Clone||KDR-2 or 260.4|
|Immunogen||Recombinant, extracellular fragment of human VEGF2.|
|Contains||15mM sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-24888 detects VEGF Receptor 2 from human samples.
MA1-24888 has been successfully used in immunohistochemistry, ELISA and Western blot procedures. The predicted molecular weight is 152 kDa.
The MA1-24888 immunogen is recombinant, extracellular fragment of human VEGF2.
Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.