|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Avian, Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Human recombinant VEGF 165.|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-21796 detects VEGF in Human, Rat and Mouse samples.
PA1-21796 has been successfully used in Immunohistochemistry (Paraffin) procedures. Staining of formalin fixed tissues requires boiling tissue sections in 10 mM Tris-HCl, pH 10.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.
PA1-21796 immunogen is Human recombinant VEGF 165.
The vascular endothelial growth factor (VEGF) family includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Anti-angiogenic effect of total saponins of Rhizoma Dioscorea nipponica on collagen induced-arthritis in rats.
PA1-21796 was used in immunohistochemistry - paraffin section to assess the therapeutic effect and mechanism of the total saponins of Rhizoma Dioscorea nipponica in rheumatoid arthritis
|Liang XJ,Guo YC,Sun TY,Song HR,Gao YX||Experimental and therapeutic medicine (12:2155)||2016|
Geraniol attenuates 2-acetylaminofluorene induced oxidative stress, inflammation and apoptosis in the liver of wistar rats.
PA1-21796 was used in immunohistochemistry - paraffin section to analyze attenuation of 2-acetylaminofluorene induced by oxidative stress, apoptosis and inflammation in the liver of wistar rats by geraniol
|Hasan SK,Sultana S||Toxicology mechanisms and methods (25:559)||2015|
Primary blast injury-induced lesions in the retina of adult rats.
PA1-21796 was used in immunohistochemistry - paraffin section to determine the effects of primary blast exposure on the retina
|Zou YY,Kan EM,Lu J,Ng KC,Tan MH,Yao L,Ling EA||Journal of neuroinflammation (10:null)||2013|
Overexpression of vascular endothelial growth factor receptor 2 in pterygia may have a predictive value for a higher postoperative recurrence rate.
PA1-21796 was used in immunohistochemistry to study the prognistic value of VEGFR-2 expression in pterygia
|Gumus K,Karakucuk S,Mirza GE,Akgun H,Arda H,Oner AO||The British journal of ophthalmology (98:796)||2014|
Sexual differences in cell proliferation in the ventricular zone, cell migration and differentiation in the HVC of juvenile Bengalese finch.
PA1-21796 was used in immunohistochemistry to study the juvenile Bengalese finch HVC and the gender-dependent differences in cell proliferation, migration and differentiation
|Chen Q,Zhang X,Zhao Y,Zhou X,Sun L,Zeng S,Zuo M,Zhang X||PloS one (9:null)||2014|
18F-fluorodeoxyglucose positron emission tomography/computed tomography and the relationship between fluorodeoxyglucose uptake and the expression of hypoxia-inducible factor-1¿, glucose transporter-1 and vascular endothelial growth factor in thymic epithelial tumours.
PA1-21796 was used in immunohistochemistry to study the the clinical value of the relationships between fluorodeoxyglucose-PET/CT and HIF-1alpha, Glut1 and VEGF in thymic epithelial tumors
|Toba H,Kondo K,Sadohara Y,Otsuka H,Morimoto M,Kajiura K,Nakagawa Y,Yoshida M,Kawakami Y,Takizawa H,Kenzaki K,Sakiyama S,Bando Y,Tangoku A||European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery (44:e105)||2013|
Angiogenesis and myogenesis in mouse tibialis anterior muscles during distraction osteogenesis: VEGF, its receptors, and myogenin genes expression.
PA1-21796 was used in immunohistochemistry to study the molecular mechanisms of angiogenesis and myogenesis in mouse tibialis anterior muscles during distraction osteogenesis
|Nishisho T,Yukata K,Matsui Y,Matsuura T,Higashino K,Suganuma K,Nikawa T,Yasui N||Journal of orthopaedic research : official publication of the Orthopaedic Research Society (30:1767)||2012|
Delayed cutaneous wound healing in aged rats compared to younger ones.
PA1-21796 was used in immunohistochemistry to study the mechanisms underlying the delayed cutaneous wound healing observed in aged rats
|Soybir OC,Gürdal SÖ,Oran E¿,Tülüba¿ F,Yüksel M,Aky¿ld¿z A¿,Bilir A,Soybir GR||International wound journal (9:478)||2012|
Dual action of TGF-ß induces vascular growth in vivo through recruitment of angiogenic VEGF-producing hematopoietic effector cells.
PA1-21796 was used in immunohistochemistry to study the significance for vascular growth of the ability of TGF-beta to both recruit hematopoietic effector cells and to induce them to express VEGF
|Fang S,Pentinmikko N,Ilmonen M,Salven P||Angiogenesis (15:511)||2012|
Effects of estrogen replacement therapy on apoptosis and vascular endothelial growth factor expression in ocular surface epithelial cells: An experimental study.
PA1-21796 was used in immunohistochemistry to study rat ocular surface apoptosis and VEGF expression following estrogen replacement therapy
|Ozcura F,Dündar SO,Cetin ED,Beder N,Dündar M||International journal of ophthalmology (5:64)||2012|
Effect of valproic acid treatment on penile structure in prepubertal rats.
PA1-21796 was used in immunohistochemistry to study the histological effects of valproic acid on the penis in prepubertal rats
|Kutlu O,Cansu A,Karagüzel E,Gürgen SG,Koç O,Gür M,Ozgür GK||Epilepsy research (99:306)||2012|
Decreased expressions of vascular endothelial growth factor and visfatin in the placental bed of pregnancies complicated by preeclampsia.
PA1-21796 was used in immunohistochemistry to study the effect of preeclampsia on the expression of VEGF and visfatin by the placental bed in the third trimester
|Kim SC,Park MJ,Joo BS,Joo JK,Suh DS,Lee KS||The journal of obstetrics and gynaecology research (38:665)||2012|
Placental chorangiosis: the association with oxidative stress and angiogenesis.
PA1-21796 was used in immunohistochemistry to study placental chorangiogiosis and the potential relationships with oxidative stress and angiogenesis
|Barut A,Barut F,Kandemir NO,Aktunc E,Arikan I,Harma M,Harma MI,Gun BD||Gynecologic and obstetric investigation (73:141)||2012|