Immunofluorescence analysis of VDR was performed using 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with VDR (9A7gamma.E10.E4) Rat Monoclonal Antibody (MA514617) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rat IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A11006) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Amphibian, Chicken, Fish, Hamster, Human, Mouse, Pig, Rat|
|Published species reactivity||Sheep, Human, Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2b|
|Immunogen||Vitamin D receptor protein from chicken intestine|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Gel Shift (GS)||1mg/mL|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14617 targets Vitamin D Receptor in GS and WB applications and shows reactivity with Amphibian, Chicken, Fish, Hamster, Human, mouse, Porcine, and Rat samples.
The MA5-14617 immunogen is vitamin D receptor protein from chicken intestine.
VDR is a member of super family of steroid hormone receptors. It is known to play role in cellular growth and differentiation, calcium and phosphorus metabolism and bone remodeling. Human breast carcinomas, leukemias, and prostatic carcinoma cells express VDR, which can modify their growth and differentiation. In avians, VDR appears as a doublet of 58kDa and 60kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Vitamin D and androgen receptor-targeted therapy for triple-negative breast cancer.
MA5-14617 was used in western blot to characterize androgen receptor-targeted and vitamin D therapy for triple-negative breast cancer
|Thakkar A,Wang B,Picon-Ruiz M,Buchwald P,Ince TA||Breast cancer research and treatment (157:77)||2016|
Crosstalk between the peroxisome proliferator-activated receptor ¿ (PPAR¿) and the vitamin D receptor (VDR) in human breast cancer cells: PPAR¿ binds to VDR and inhibits 1¿,25-dihydroxyvitamin D3 mediated transactivation.
MA5-14617 was used in western blot to study the functional consequences of PPAR-gamma-VDR cross-talk in human breast cancer cells
|Alimirah F,Peng X,Yuan L,Mehta RR,von Knethen A,Choubey D,Mehta RG||Experimental cell research (318:2490)||2012|
Chemotherapeutic effect of calcidiol derivative B3CD in a neuroblastoma xenograft model.
MA5-14617 was used in western blot to investigate the effect of bromoacetoxy-calcidiol on neuroblastoma cells and its potential therapeutic applications in vivo
|Lange TS,Zou Y,Singh RK,Kim KK,Kristjansdottir K,Sholler GL,Brard L||Chemical biology and drug design (76:164)||2010|
Characterization of mammary tumor cell lines from wild type and vitamin D3 receptor knockout mice.
MA5-14617 was used in western blot to characterize mammary tumor cell lines from wild type and vitamin D3 receptor knockout mice
|Zinser GM,McEleney K,Welsh J||Molecular and cellular endocrinology (200:67)||2003|
Induction of apoptosis by 1,25-dihydroxyvitamin D3 in MCF-7 Vitamin D3-resistant variant can be sensitized by TPA.
MA5-14617 was used in western blot to investigate the effect of TPA on the apoptosis induced by 1,25-dihydroxyvitamin D3 in MCF-7 vitamin D3-resistant variant
|Narvaez CJ,Byrne BM,Romu S,Valrance M,Welsh J||The Journal of steroid biochemistry and molecular biology (84:199)||2003|
Mitochondrial translocation of vitamin D receptor is mediated by the permeability transition pore in human keratinocyte cell line.
MA5-14617 was used in immunoprecipitation to study the role of the permeability transition pore in the mitochondrial translocation of the vitamin D receptor in human keratinocytes
|Silvagno F,Consiglio M,Foglizzo V,Destefanis M,Pescarmona G||PloS one (8:null)||2013|
Influence of different calcium supplies and a single vitamin D injection on vitamin D receptor and calbindin D9k immunoreactivities in the gastrointestinal tract of goat kids.
MA5-14617 was used in immunohistochemistry to study the effect of Ca2+ concentration on vitamin D receptor and calbindin D9k immunoreactivities in the gastrointestinal tract of growing goats
|Sidler-Lauff K,Boos A,Kraenzlin M,Liesegang A||Journal of animal science (88:3598)||2010|
1,25 Dihydroxyvitamin D(3) receptor expression in superficial transitional cell carcinoma of the bladder: a possible prognostic factor?
MA5-14617 was used in immunohistochemistry to investigate the prognostic value of vitamin D receptors in superficial transitional cell carcinoma of the human bladder
|Sahin MO,Canda AE,Yorukoglu K,Mungan MU,Sade M,Kirkali Z||European urology (47:52)||2005|