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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide from the C-terminus of human Wilm's Tumor protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is Wilm's tumor tissue.
Wilm"e;s Tumor (WT), a sporadic and familial childhood kidney tumor, is genetically heterogeneous. Wilm"e;s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelioma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
amino-terminal domain of EWS; AWT1; EWS-WT1; GUD; last three zinc fingers of the DNA-binding domain of WT1; NPHS4; WAGR; Wilms tumor protein isoform Ex4a(+); WIT-2; WT
AWT1; EWS-WT1; GUD; NPHS4; WAGR; WIT-2; WT1; WT33