|Tested species reactivity||Human|
|Published species reactivity||Bovine, Human|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Recombinant human XPA protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
MA5-13835 targets XPA in IHC (P) and WB applications and shows reactivity with Human samples.
The MA5-13835 immunogen is recombinant human XPA protein.
The XPA (xeroderma pigmentosum group A) protein specifically recognizes the UV-or chemically damaged DNA lesions, and triggers the nucleotide excision repair process. XPA binds to the replication protein A (RPA) or the excision repair cross complementing 1 protein (ERCC1). In the absence of nucleotide excision repair persisting (unrepaired) DNA lesions (adducts) may lead to the accumulation of gene mutations and ultimately to cancer. Xeroderma pigmentosum patients have a > 2000 fold increased risk to develop skin cancer at sun-exposed areas.
ATR pathway inhibition is synthetically lethal in cancer cells with ERCC1 deficiency.
MA5-13835 was used in western blot to study the the synthetic lethality of ATR inhibitors in cells deficient for ERCC1 and the implications for cancer therapy
|Mohni KN,Kavanaugh GM,Cortez D||Cancer research (74:2835)||2014|
Recognition of cisplatin-DNA interstrand cross-links by replication protein A.
MA5-13835 was used in western blot to study the ability of replication protein to recognize and bind to cisplatin-DNA interstrand cross-links
|Patrick SM,Tillison K,Horn JM||Biochemistry (47:10188)||2008|
Molecular characterization of spontaneous mesenchymal stem cell transformation.
MA5-13835 was used in western blot to investigate the molecular mechanisms of spontaneous mesenchymal stem cell transformation
|Rubio D,Garcia S,Paz MF,De la Cueva T,Lopez-Fernandez LA,Lloyd AC,Garcia-Castro J,Bernad A||PloS one (3:null)||2008|
A rapid assay for measuring nucleotide excision repair by oligonucleotide retrieval.
MA5-13835 was used in blocking or activating experiment to develop an assay for the sensitive and quantitative measuremt of nucleotide excision repair activity in a variety of human cell types
|Shen JC,Fox EJ,Ahn EH,Loeb LA||Scientific reports (4:null)||2014|
Physiological modulation of endogenous BRCA1 p220 abundance suppresses DNA damage during the cell cycle.
MA5-13835 was used in immunocytochemistry and western blot to study the importance of correct physiological regulation of BRCA1 p220 levels in maintaining genome stability through the cell cycle
|Dimitrov SD,Lu D,Naetar N,Hu Y,Pathania S,Kanellopoulou C,Livingston DM||Genes & development (27:2274)||2013|
Lack of CAK complex accumulation at DNA damage sites in XP-B and XP-B/CS fibroblasts reveals differential regulation of CAK anchoring to core TFIIH by XPB and XPD helicases during nucleotide excision repair.
MA5-13835 was used in immunocytochemistry to study the role of the XPB and XPD helicases in regulating the anchorage of the CAK complex to TFIIH during nucleotide excision repair
|Zhu Q,Wani G,Sharma N,Wani A||DNA repair (11:942)||2012|
Dissociation of CAK from core TFIIH reveals a functional link between XP-G/CS and the TFIIH disassembly state.
MA5-13835 was used in immunocytochemistry to study the functional association between XP-G/CS mutations and the disassembly state of TFIIH
|Arab HH,Wani G,Ray A,Shah ZI,Zhu Q,Wani AA||PloS one (5:null)||2010|
Chromatin restoration following nucleotide excision repair involves the incorporation of ubiquitinated H2A at damaged genomic sites.
MA5-13835 was used in immunocytochemistry to study the role of ubiquitinated H2A in chromatin restoration after nucleotide excision repair
|Zhu Q,Wani G,Arab HH,El-Mahdy MA,Ray A,Wani AA||DNA repair (8:262)||2009|
Functional implications of antiestrogen induction of quinone reductase: inhibition of estrogen-induced deoxyribonucleic acid damage.
MA5-13835 was used in immunocytochemistry to study the role of quinone reductase induction by anti-estrogens in protecting against oxidative DNA damage by estrogen metabolites
|Bianco NR,Perry G,Smith MA,Templeton DJ,Montano MM||Molecular endocrinology (Baltimore, Md.) (17:1344)||2003|
DNA polymerase epsilon associates with the elongating form of RNA polymerase II and nascent transcripts.
MA5-13835 was used in immunoprecipitation to study the interaction of DNA polymerase epsilon with the elongating form of RNA polymerase II
|Rytkönen AK,Hillukkala T,Vaara M,Sokka M,Jokela M,Sormunen R,Nasheuer HP,Nethanel T,Kaufmann G,Pospiech H,Syväoja JE||The FEBS journal (273:5535)||2006|
Expression of xeroderma pigmentosum A protein predicts improved outcome in metastatic ovarian carcinoma.
MA5-13835 was used in immunohistochemistry to evaluate the prognostic value of xeroderma pigmentosum A protein expression in metastatic ovarian carcinoma
|Stevens EV,Raffeld M,Espina V,Kristensen GB,Trope' CG,Kohn EC,Davidson B||Cancer (103:2313)||2005|
excision repair-controlling; mutant xeroderma pigmentosum complementation group A; xeroderma pigmentosum group A-complementing protein; xeroderma pigmentosum, complementation group A
XP1; XPA; XPAC