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|Tested species reactivity||Human|
|Published species reactivity||Cattle , Human|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Recombinant human XPA protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13835 targets XPA in IHC (P) and WB applications and shows reactivity with Human samples.
The MA5-13835 immunogen is recombinant human XPA protein.
The XPA (xeroderma pigmentosum group A) protein specifically recognizes the UV-or chemically damaged DNA lesions, and triggers the nucleotide excision repair process. XPA binds to the replication protein A (RPA) or the excision repair cross complementing 1 protein (ERCC1). In the absence of nucleotide excision repair persisting (unrepaired) DNA lesions (adducts) may lead to the accumulation of gene mutations and ultimately to cancer. Xeroderma pigmentosum patients have a > 2000 fold increased risk to develop skin cancer at sun-exposed areas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ATR pathway inhibition is synthetically lethal in cancer cells with ERCC1 deficiency.
MA5-13835 was used in western blot to study the the synthetic lethality of ATR inhibitors in cells deficient for ERCC1 and the implications for cancer therapy
|Mohni KN,Kavanaugh GM,Cortez D||Cancer research (74:2835)||2014|
Recognition of cisplatin-DNA interstrand cross-links by replication protein A.
MA5-13835 was used in western blot to study the ability of replication protein to recognize and bind to cisplatin-DNA interstrand cross-links
|Patrick SM,Tillison K,Horn JM||Biochemistry (47:10188)||2008|
Molecular characterization of spontaneous mesenchymal stem cell transformation.
MA5-13835 was used in western blot to investigate the molecular mechanisms of spontaneous mesenchymal stem cell transformation
|Rubio D,Garcia S,Paz MF,De la Cueva T,Lopez-Fernandez LA,Lloyd AC,Garcia-Castro J,Bernad A||PloS one (3:null)||2008|
A rapid assay for measuring nucleotide excision repair by oligonucleotide retrieval.
MA5-13835 was used in blocking or activating experiment to develop an assay for the sensitive and quantitative measuremt of nucleotide excision repair activity in a variety of human cell types
|Shen JC,Fox EJ,Ahn EH,Loeb LA||Scientific reports (4:null)||2014|
Physiological modulation of endogenous BRCA1 p220 abundance suppresses DNA damage during the cell cycle.
MA5-13835 was used in immunocytochemistry and western blot to study the importance of correct physiological regulation of BRCA1 p220 levels in maintaining genome stability through the cell cycle
|Dimitrov SD,Lu D,Naetar N,Hu Y,Pathania S,Kanellopoulou C,Livingston DM||Genes & development (27:2274)||2013|
Lack of CAK complex accumulation at DNA damage sites in XP-B and XP-B/CS fibroblasts reveals differential regulation of CAK anchoring to core TFIIH by XPB and XPD helicases during nucleotide excision repair.
MA5-13835 was used in immunocytochemistry to study the role of the XPB and XPD helicases in regulating the anchorage of the CAK complex to TFIIH during nucleotide excision repair
|Zhu Q,Wani G,Sharma N,Wani A||DNA repair (11:942)||2012|
Dissociation of CAK from core TFIIH reveals a functional link between XP-G/CS and the TFIIH disassembly state.
MA5-13835 was used in immunocytochemistry to study the functional association between XP-G/CS mutations and the disassembly state of TFIIH
|Arab HH,Wani G,Ray A,Shah ZI,Zhu Q,Wani AA||PloS one (5:null)||2010|
Chromatin restoration following nucleotide excision repair involves the incorporation of ubiquitinated H2A at damaged genomic sites.
MA5-13835 was used in immunocytochemistry to study the role of ubiquitinated H2A in chromatin restoration after nucleotide excision repair
|Zhu Q,Wani G,Arab HH,El-Mahdy MA,Ray A,Wani AA||DNA repair (8:262)||2009|
Functional implications of antiestrogen induction of quinone reductase: inhibition of estrogen-induced deoxyribonucleic acid damage.
MA5-13835 was used in immunocytochemistry to study the role of quinone reductase induction by anti-estrogens in protecting against oxidative DNA damage by estrogen metabolites
|Bianco NR,Perry G,Smith MA,Templeton DJ,Montano MM||Molecular endocrinology (Baltimore, Md.) (17:1344)||2003|
DNA polymerase epsilon associates with the elongating form of RNA polymerase II and nascent transcripts.
MA5-13835 was used in immunoprecipitation to study the interaction of DNA polymerase epsilon with the elongating form of RNA polymerase II
|Rytkönen AK,Hillukkala T,Vaara M,Sokka M,Jokela M,Sormunen R,Nasheuer HP,Nethanel T,Kaufmann G,Pospiech H,Syväoja JE||The FEBS journal (273:5535)||2006|
Expression of xeroderma pigmentosum A protein predicts improved outcome in metastatic ovarian carcinoma.
MA5-13835 was used in immunohistochemistry to evaluate the prognostic value of xeroderma pigmentosum A protein expression in metastatic ovarian carcinoma
|Stevens EV,Raffeld M,Espina V,Kristensen GB,Trope' CG,Kohn EC,Davidson B||Cancer (103:2313)||2005|
DNA repair protein complementing XP-A cells, excision repair-controlling, xeroderma pigmentosum group A-complementing protein