Invitrogen

XPA Monoclonal Antibody (12F5)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

6 Published Figures

14 References

View all (16) XPA antibodies

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Datasheet

Protocols

Questions & Answers

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XPA Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of XPA was performed using 70 % confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with XPA (12F5) Mouse Monoclonal antibody (Product # MA5-13835) at 5 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

XPA Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Formalin-fixed, paraffin-embedded human tonsil stained with XPA antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of epithelial cells.

XPA Antibody in Western Blot (WB) — Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of A-431 (Lane 1), Jurkat (Lane 2), HeLa (Lane 3), MCF7 (Lane 4), K-562 (Lane 5), Hep G2 (Lane 6), Raji (Lane 7), HCT 116 (Lane 8), and PANC-1 (Lane 9). The blots were probed with Mouse Anti-XPA Monoclonal Antibody (Product # MA5-13835, 1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 37 kDa band corresponding to XPA was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

XPA Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A431 cells were transfected with XPA siRNA and decrease in signal intensity was observed in western blot application using anti-XPA siRNA (Product # MA5-13835). {KD}

Product Details

MA5-13835

Applications	Tested Dilution	Publications
Western Blot (WB)	1-2 µg/mL	View 5 publications 5 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	2-4 µg/mL	-
Immunocytochemistry (ICC/IF)	5 µg/mL	View 6 publications 6 publications
Immunoprecipitation (IP)	-	View 1 publication 1 publication
Neutralization (Neu)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Bovine, Human
Host/Isotype	Mouse / IgG2a, kappa
Class	Monoclonal
Type	Antibody
Clone	12F5
Immunogen	Recombinant human XPA protein
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_10985162

Product Specific Information

MA5-13835 targets XPA in IHC (P) and WB applications and shows reactivity with Human samples.

The MA5-13835 immunogen is recombinant human XPA protein.

Target Information

The XPA (xeroderma pigmentosumgroup A) protein specifically recognizes the UV-orchemically damaged DNA lesions, and triggers thenucleotide excision repair process. XPA binds to thereplication protein A (RPA) or the excision repaircross complementing 1 protein (ERCC 1). In the absence of nucleotide excision repair persisting (unrepaired) DNA lesions (adducts) may lead to the accumulation of gene mutations and ultimately to cancer. Xeroderma pigmentosum patients have a >2000 fold increased risk to develop skin cancer atsun-exposed areas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Elevated glucose increases genomic instability by inhibiting nucleotide excision repair.

Life science alliance

Ciminera AK,Shuck SC,Termini J

Published figure using XPA monoclonal antibody (Product # MA5-13835) in Western Blot

Fri Oct 01 00:00:00 EDT 2021

Progerin sequestration of PCNA promotes replication fork collapse and mislocalization of XPA in laminopathy-related progeroid syndromes.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Hilton BA,Liu J,Cartwright BM,Liu Y,Breitman M,Wang Y,Jones R,Tang H,Rusinol A,Musich PR,Zou Y

Published figure using XPA monoclonal antibody (Product # MA5-13835) in Western Blot

Fri Sep 01 00:00:00 EDT 2017

ATR pathway inhibition is synthetically lethal in cancer cells with ERCC1 deficiency.

Cancer research

Mohni KN,Kavanaugh GM,Cortez D

MA5-13835 was used in western blot to study the the synthetic lethality of ATR inhibitors in cells deficient for ERCC1 and the implications for cancer therapy

Thu May 15 00:00:00 EDT 2014

A rapid assay for measuring nucleotide excision repair by oligonucleotide retrieval.

Scientific reports

Shen JC,Fox EJ,Ahn EH,Loeb LA

MA5-13835 was used in blocking or activating experiment to develop an assay for the sensitive and quantitative measuremt of nucleotide excision repair activity in a variety of human cell types

Thu May 08 00:00:00 EDT 2014

Physiological modulation of endogenous BRCA1 p220 abundance suppresses DNA damage during the cell cycle.

Genes & development

Dimitrov SD,Lu D,Naetar N,Hu Y,Pathania S,Kanellopoulou C,Livingston DM

MA5-13835 was used in immunocytochemistry and western blot to study the importance of correct physiological regulation of BRCA1 p220 levels in maintaining genome stability through the cell cycle

Tue Oct 15 00:00:00 EDT 2013

Lack of CAK complex accumulation at DNA damage sites in XP-B and XP-B/CS fibroblasts reveals differential regulation of CAK anchoring to core TFIIH by XPB and XPD helicases during nucleotide excision repair.

DNA repair

Zhu Q,Wani G,Sharma N,Wani A

MA5-13835 was used in immunocytochemistry to study the role of the XPB and XPD helicases in regulating the anchorage of the CAK complex to TFIIH during nucleotide excision repair

Sat Dec 01 00:00:00 EST 2012

Sodium arsenite and hyperthermia modulate cisplatin-DNA damage responses and enhance platinum accumulation in murine metastatic ovarian cancer xenograft after hyperthermic intraperitoneal chemotherapy (HIPEC).

Journal of ovarian research

Muenyi CS,States VA,Masters JH,Fan TW,Helm CW,States JC

MA5-13835 was used in immunocytochemistry to report that sodium arsenite and hyperthermia modulate cisplatin-DNA damage responses and enhance platinum accumulation in murine metastatic ovarian cancer xenograft after hyperthermic intraperitoneal chemothera

Wed Jun 22 00:00:00 EDT 2011

Dissociation of CAK from core TFIIH reveals a functional link between XP-G/CS and the TFIIH disassembly state.

PloS one

Arab HH,Wani G,Ray A,Shah ZI,Zhu Q,Wani AA

MA5-13835 was used in immunocytochemistry to study the functional association between XP-G/CS mutations and the disassembly state of TFIIH

Tue Jun 08 00:00:00 EDT 2010

Chromatin restoration following nucleotide excision repair involves the incorporation of ubiquitinated H2A at damaged genomic sites.

DNA repair

Zhu Q,Wani G,Arab HH,El-Mahdy MA,Ray A,Wani AA

MA5-13835 was used in immunocytochemistry to study the role of ubiquitinated H2A in chromatin restoration after nucleotide excision repair

Sun Feb 01 00:00:00 EST 2009

Recognition of cisplatin-DNA interstrand cross-links by replication protein A.

Biochemistry

Patrick SM,Tillison K,Horn JM

MA5-13835 was used in western blot to study the ability of replication protein to recognize and bind to cisplatin-DNA interstrand cross-links

Tue Sep 23 00:00:00 EDT 2008

Molecular characterization of spontaneous mesenchymal stem cell transformation.

PloS one

Rubio D,Garcia S,Paz MF,De la Cueva T,Lopez-Fernandez LA,Lloyd AC,Garcia-Castro J,Bernad A

MA5-13835 was used in western blot to investigate the molecular mechanisms of spontaneous mesenchymal stem cell transformation

Wed Jan 02 00:00:00 EST 2008

DNA polymerase epsilon associates with the elongating form of RNA polymerase II and nascent transcripts.

The FEBS journal

Rytkönen AK,Hillukkala T,Vaara M,Sokka M,Jokela M,Sormunen R,Nasheuer HP,Nethanel T,Kaufmann G,Pospiech H,Syväoja JE

MA5-13835 was used in immunoprecipitation to study the interaction of DNA polymerase epsilon with the elongating form of RNA polymerase II

Fri Dec 01 00:00:00 EST 2006

Expression of xeroderma pigmentosum A protein predicts improved outcome in metastatic ovarian carcinoma.

Cancer

Stevens EV,Raffeld M,Espina V,Kristensen GB,Trope' CG,Kohn EC,Davidson B

MA5-13835 was used in immunohistochemistry to evaluate the prognostic value of xeroderma pigmentosum A protein expression in metastatic ovarian carcinoma

Wed Jun 01 00:00:00 EDT 2005

Functional implications of antiestrogen induction of quinone reductase: inhibition of estrogen-induced deoxyribonucleic acid damage.

Molecular endocrinology (Baltimore, Md.)

Bianco NR,Perry G,Smith MA,Templeton DJ,Montano MM

MA5-13835 was used in immunocytochemistry to study the role of quinone reductase induction by anti-estrogens in protecting against oxidative DNA damage by estrogen metabolites

Tue Jul 01 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: DNA repair protein complementing XP-A cells; excision repair-controlling; mutant xeroderma pigmentosum complementation group A; Xeroderma pigmentosum group A-complementing protein; xeroderma pigmentosum, complementation group A

Gene Aliases: XP1; XPA; XPAC

UniProt ID: (Human) P23025

Entrez Gene ID: (Human) 7507

Molecular Function: damaged DNA-binding protein

Function(s)

Process(es)

damaged DNA binding protein binding protein domain specific binding protein homodimerization activity metal ion binding

XPA Monoclonal Antibody (12F5)

Product Details

MA5-13835

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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